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NFIA Antibody (monoclonal) (M02)

Mouse monoclonal antibody raised against a full length recombinant NFIA.

  • WB - NFIA Antibody (monoclonal) (M02) AT3032a
    Antibody Reactive Against Recombinant Protein.Western Blot detection against Immunogen (80.52 KDa) .
  • WB - NFIA Antibody (monoclonal) (M02) AT3032a
    Western Blot analysis of NFIA expression in transfected 293T cell line by NFIA monoclonal antibody (M02), clone 1E11.

    Lane 1: NFIA transfected lysate (Predicted MW: 54.6 KDa).
    Lane 2: Non-transfected lysate.
  • IF - NFIA Antibody (monoclonal) (M02) AT3032a
    Immunofluorescence of monoclonal antibody to NFIA on HeLa cell . [antibody concentration 10 ug/ml]
  • E - NFIA Antibody (monoclonal) (M02) AT3032a
    Detection limit for recombinant GST tagged NFIA is approximately 0.3ng/ml as a capture antibody.
Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary Accession Q12857
Other Accession BC022264
Reactivity Human
Host mouse
Clonality Monoclonal
Isotype IgG1 Kappa
Clone Names 1E12
Calculated MW 55944 Da
Additional Information
Gene ID 4774
Other Names Nuclear factor 1 A-type, NF1-A, Nuclear factor 1/A, CCAAT-box-binding transcription factor, CTF, Nuclear factor I/A, NF-I/A, NFI-A, TGGCA-binding protein, NFIA, KIAA1439
Target/Specificity NFIA (AAH22264, 1 a.a. ~ 498 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
Dilution WB~~1:500~1000
Format Clear, colorless solution in phosphate buffered saline, pH 7.2 .
StorageStore at -20°C or lower. Aliquot to avoid repeated freezing and thawing.
PrecautionsNFIA Antibody (monoclonal) (M02) is for research use only and not for use in diagnostic or therapeutic procedures.
Research Areas
Citations (0)

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Nuclear factor I (NFI) proteins constitute a family of dimeric DNA-binding proteins with similar, and possibly identical, DNA-binding specificity. They function as cellular transcription factors and as replication factors for adenovirus DNA replication. Diversity in this protein family is generated by multiple genes, differential splicing, and heterodimerization.


1.The interplay between the master transcription factor PU.1 and miR-424 regulates human monocyte/macrophage differentiation.Rosa A, Ballarino M, Sorrentino A, Sthandier O, De Angelis FG, Marchioni M, Masella B, Guarini A, Fatica A, Peschle C, Bozzoni I.Proc Natl Acad Sci U S A. 2007 Dec 11;104(50):19849-54. Epub 2007 Dec 3.

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$ 315.00
Cat# AT3032a
(40 western blots)
Availability: 7-10 days
Bulk Size
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