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NFKB1 Antibody (monoclonal) (M01)

Mouse monoclonal antibody raised against a partial recombinant NFKB1.

  • WB - NFKB1 Antibody (monoclonal) (M01) AT3035a
    Antibody Reactive Against Recombinant Protein.Western Blot detection against Immunogen (37.73 KDa) .
  • WB - NFKB1 Antibody (monoclonal) (M01) AT3035a
    Western Blot analysis of NFKB1 expression in transfected 293T cell line by NFKB1 monoclonal antibody (M01), clone 2E6.

    Lane 1: NFKB1 transfected lysate(105.4 KDa).
    Lane 2: Non-transfected lysate.
  • IF - NFKB1 Antibody (monoclonal) (M01) AT3035a
    Immunofluorescence of monoclonal antibody to NFKB1 on HeLa cell. [antibody concentration 10 ug/ml]
  • E - NFKB1 Antibody (monoclonal) (M01) AT3035a
    Detection limit for recombinant GST tagged NFKB1 is approximately 10ng/ml as a capture antibody.
  • WB - NFKB1 Antibody (monoclonal) (M01) AT3035a
    Western blot analysis of NFKB1 over-expressed 293 cell line, cotransfected with NFKB1 Validated Chimera RNAi ( (Cat # AT3035a )
Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary Accession P19838
Other Accession BC051765
Reactivity Human
Host mouse
Clonality Monoclonal
Isotype IgG1 kappa
Clone Names 2E7
Calculated MW 105356 Da
Additional Information
Gene ID 4790
Other Names Nuclear factor NF-kappa-B p105 subunit, DNA-binding factor KBF1, EBP-1, Nuclear factor of kappa light polypeptide gene enhancer in B-cells 1, Nuclear factor NF-kappa-B p50 subunit, NFKB1
Target/Specificity NFKB1 (AAH51765, 860 a.a. ~ 969 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
Dilution WB~~1:500~1000
Format Clear, colorless solution in phosphate buffered saline, pH 7.2 .
StorageStore at -20°C or lower. Aliquot to avoid repeated freezing and thawing.
PrecautionsNFKB1 Antibody (monoclonal) (M01) is for research use only and not for use in diagnostic or therapeutic procedures.
Research Areas
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This gene encodes a 105 kD protein which can undergo cotranslational processing by the 26S proteasome to produce a 50 kD protein. The 105 kD protein is a Rel protein-specific transcription inhibitor and the 50 kD protein is a DNA binding subunit of the NF-kappa-B (NFKB) protein complex. NFKB is a transcription regulator that is activated by various intra- and extra-cellular stimuli such as cytokines, oxidant-free radicals, ultraviolet irradiation, and bacterial or viral products. Activated NFKB translocates into the nucleus and stimulates the expression of genes involved in a wide variety of biological functions. Inappropriate activation of NFKB has been associated with a number of inflammatory diseases while persistent inhibition of NFKB leads to inappropriate immune cell development or delayed cell growth. Two transcript variants encoding different isoforms have been found for this gene.


Posttonsillectomy hemorrhage: blame on surgeons or genes? Arweiler-Harbeck D, et al. Laryngoscope, 2010 Sep. PMID 20715085.A genetic association study of maternal and fetal candidate genes that predispose to preterm prelabor rupture of membranes (PROM). Romero R, et al. Am J Obstet Gynecol, 2010 Jul 29. PMID 20673868.A large-scale candidate gene approach identifies SNPs in SOD2 and IL13 as predictive markers of response to preoperative chemoradiation in rectal cancer. Ho-Pun-Cheung A, et al. Pharmacogenomics J, 2010 Jul 20. PMID 20644561.Regulation of NF-kappaB activity and inducible nitric oxide synthase by regulatory particle non-ATPase subunit 13 (Rpn13). Mazumdar T, et al. Proc Natl Acad Sci U S A, 2010 Aug 3. PMID 20634424.Variation at the NFATC2 Locus Increases the Risk of Thiazolinedinedione-Induced Edema in the Diabetes REduction Assessment with ramipril and rosiglitazone Medication (DREAM) Study. Bailey SD, et al. Diabetes Care, 2010 Jul 13. PMID 20628086.

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$ 315.00
Cat# AT3035a
(40 western blots)
Availability: 7-10 days
Bulk Size
Seasonal Special on Bulk Order
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