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NFYB Antibody (monoclonal) (M03)

Mouse monoclonal antibody raised against a full length recombinant NFYB.

  • WB - NFYB Antibody (monoclonal) (M03) AT3042a
    Antibody Reactive Against Recombinant Protein.Western Blot detection against Immunogen (48.51 KDa) .
  • IF - NFYB Antibody (monoclonal) (M03) AT3042a
    Immunofluorescence of monoclonal antibody to NFYB on HeLa cell . [antibody concentration 10 ug/ml]
  • E - NFYB Antibody (monoclonal) (M03) AT3042a
    Detection limit for recombinant GST tagged NFYB is approximately 0.03ng/ml as a capture antibody.
Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary Accession P25208
Other Accession BC005317
Reactivity Human
Host mouse
Clonality Monoclonal
Isotype IgG2a Kappa
Clone Names 5D1
Calculated MW 22831 Da
Additional Information
Gene ID 4801
Other Names Nuclear transcription factor Y subunit beta, CAAT box DNA-binding protein subunit B, Nuclear transcription factor Y subunit B, NF-YB, NFYB, HAP3
Target/Specificity NFYB (AAH05317, 1 a.a. ~ 207 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
Dilution WB~~1:500~1000
Format Clear, colorless solution in phosphate buffered saline, pH 7.2 .
StorageStore at -20°C or lower. Aliquot to avoid repeated freezing and thawing.
PrecautionsNFYB Antibody (monoclonal) (M03) is for research use only and not for use in diagnostic or therapeutic procedures.
Research Areas
Citations (0)

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The protein encoded by this gene is one subunit of a trimeric complex, forming a highly conserved transcription factor that binds with high specificity to CCAAT motifs in the promoter regions in a variety of genes. This gene product, subunit B, forms a tight dimer with the C subunit, a prerequisite for subunit A association. The resulting trimer binds to DNA with high specificity and affinity. Subunits B and C each contain a histone-like motif. Observation of the histone nature of these subunits is supported by two types of evidence; protein sequence alignments and experiments with mutants.


HMGB1 and HMGB2 proteins up-regulate cellular expression of human topoisomerase IIalpha. Stros M, et al. Nucleic Acids Res, 2009 Apr. PMID 19223331.In cultured oligodendrocytes the A/B-type hnRNP CBF-A accompanies MBP mRNA bound to mRNA trafficking sequences. Raju CS, et al. Mol Biol Cell, 2008 Jul. PMID 18480411.CCAAT box is required for the induction of human thrombospondin-1 gene by trichostatin A. Kang JH, et al. J Cell Biochem, 2008 Jul 1. PMID 18275041.Gain of function of mutant p53: the mutant p53/NF-Y protein complex reveals an aberrant transcriptional mechanism of cell cycle regulation. Di Agostino S, et al. Cancer Cell, 2006 Sep. PMID 16959611.Diversification of transcriptional modulation: large-scale identification and characterization of putative alternative promoters of human genes. Kimura K, et al. Genome Res, 2006 Jan. PMID 16344560.

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$ 315.00
Cat# AT3042a
(40 western blots)
Availability: 7-10 days
Bulk Size
Seasonal Special on Bulk Order
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