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NPM1 Antibody (monoclonal) (M01)

Mouse monoclonal antibody raised against a full length recombinant NPM1.

  • WB - NPM1 Antibody (monoclonal) (M01) AT3085a
    Antibody Reactive Against Recombinant Protein.Western Blot detection against Immunogen (58.08 KDa) .
  • WB - NPM1 Antibody (monoclonal) (M01) AT3085a
    NPM1 monoclonal antibody (M01), clone 3B2 Western Blot analysis of NPM1 expression in Hela S3 NE ( (Cat # AT3085a )
  • IHC - NPM1 Antibody (monoclonal) (M01) AT3085a
    Immunoperoxidase of monoclonal antibody to NPM1 on formalin-fixed paraffin-embedded human ovary, clear cell carcinoma tissue. [antibody concentration 5 ug/ml]
  • IF - NPM1 Antibody (monoclonal) (M01) AT3085a
    Immunofluorescence of monoclonal antibody to NPM1 on HeLa cell. [antibody concentration 10 ug/ml]
  • E - NPM1 Antibody (monoclonal) (M01) AT3085a
    Detection limit for recombinant GST tagged NPM1 is 0.3 ng/ml as a capture antibody.
Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary Accession P06748
Other Accession BC002398
Reactivity Human
Host mouse
Clonality Monoclonal
Isotype IgG1 kappa
Clone Names 3B2
Calculated MW 32575 Da
Additional Information
Gene ID 4869
Other Names Nucleophosmin, NPM, Nucleolar phosphoprotein B23, Nucleolar protein NO38, Numatrin, NPM1, NPM
Target/Specificity NPM1 (AAH02398.1, 1 a.a. ~ 294 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
Dilution WB~~1:500~1000
Format Clear, colorless solution in phosphate buffered saline, pH 7.2 .
StorageStore at -20°C or lower. Aliquot to avoid repeated freezing and thawing.
PrecautionsNPM1 Antibody (monoclonal) (M01) is for research use only and not for use in diagnostic or therapeutic procedures.
Citations (0)

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This gene encodes a phosphoprotein which moves between the nucleus and the cytoplasm. The gene product is thought to be involved in several processes including regulation of the ARF/p53 pathway. A number of genes are fusion partners have been characterized, in particular the anaplastic lymphoma kinase gene on chromosome 2. Mutations in this gene are associated with acute myeloid leukemia. More than a dozen pseudogenes of this gene have been identified. Alternative splicing results in multiple transcript variants.


Incidence and significance of FLT3-ITD and NPM1 mutations in patients with normal karyotype acute myeloid leukaemia. Haslam K, et al. Ir J Med Sci, 2010 Aug 31. PMID 20803351.Combined mutations of ASXL1, CBL, FLT3, IDH1, IDH2, JAK2, KRAS, NPM1, NRAS, RUNX1, TET2 and WT1 genes in myelodysplastic syndromes and acute myeloid leukemias. Rocquain J, et al. BMC Cancer, 2010 Aug 2. PMID 20678218.The prognostic significance of IDH1 mutations in younger adult patients with acute myeloid leukemia is dependent on FLT3/ITD status. Green CL, et al. Blood, 2010 Jul 22. PMID 20651067.Performance and clinical evaluation of a sensitive multiplex assay for the rapid detection of common NPM1 mutations. Hafez M, et al. J Mol Diagn, 2010 Sep. PMID 20616361.High frequency type I/II mutational shifts between diagnosis and relapse are associated with outcome in pediatric AML: implications for personalized medicine. Bachas C, et al. Blood, 2010 Jun 30. PMID 20592250.

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$ 315.00
Cat# AT3085a
(40 western blots)
Availability: 7-10 days
Bulk Size
Seasonal Special on Bulk Order
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