NT5C2 Antibody (monoclonal) (M02)
Mouse monoclonal antibody raised against a full length recombinant NT5C2.
|Application ||WB, IHC, IF|
|Calculated MW||64970 Da|
|Other Names||Cytosolic purine 5'-nucleotidase, Cytosolic 5'-nucleotidase II, NT5C2, NT5B, NT5CP, PNT5|
|Target/Specificity||NT5C2 (AAH01595, 1 a.a. ~ 561 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.|
|Format||Clear, colorless solution in phosphate buffered saline, pH 7.2 .|
|Storage||Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.|
|Precautions||NT5C2 Antibody (monoclonal) (M02) is for research use only and not for use in diagnostic or therapeutic procedures.|
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Provided below are standard protocols that you may find useful for product applications.
Purine 5-prime-nucleotidase (EC 22.214.171.124) preferentially hydrolyzes inosine 5-prime-monophosphate (IMP) and other purine nucleotides, and is allosterically activated by various compounds, including ATP. The enzyme is exclusively located in the cytoplasmic matrix of cells and may have a critical role in the maintenance of a constant composition of intracellular purine/pyrimidine nucleotides in cooperation with other nucleotidases.
1.Suppression of 5'-Nucleotidase Enzymes Promotes AMP-activated Protein Kinase (AMPK) Phosphorylation and Metabolism in Human and Mouse Skeletal Muscle.Kulkarni SS, Karlsson HK, Szekeres F, Chibalin AV, Krook A, Zierath JR.J Biol Chem. 2011 Oct 7;286(40):34567-74. Epub 2011 Aug 26.
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