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NUDT21 Antibody (monoclonal) (M12)

Mouse monoclonal antibody raised against a full length recombinant NUDT21.

  • WB - NUDT21 Antibody (monoclonal) (M12) AT3130a
    Antibody Reactive Against Recombinant Protein.Western Blot detection against Immunogen (50.71 KDa) .
  • IHC - NUDT21 Antibody (monoclonal) (M12) AT3130a
    Immunoperoxidase of monoclonal antibody to NUDT21 on formalin-fixed paraffin-embedded human testis. [antibody concentration 3 ug/ml]
  • IF - NUDT21 Antibody (monoclonal) (M12) AT3130a
    Immunofluorescence of monoclonal antibody to NUDT21 on HeLa cell . [antibody concentration 10 ug/ml]
  • E - NUDT21 Antibody (monoclonal) (M12) AT3130a
    Detection limit for recombinant GST tagged NUDT21 is approximately 3ng/ml as a capture antibody.
Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary Accession O43809
Other Accession BC001403
Reactivity Human
Host mouse
Clonality Monoclonal
Isotype IgG1 Kappa
Clone Names 3F8
Calculated MW 26227 Da
Additional Information
Gene ID 11051
Other Names Cleavage and polyadenylation specificity factor subunit 5, Cleavage and polyadenylation specificity factor 25 kDa subunit, CFIm25, CPSF 25 kDa subunit, Nucleoside diphosphate-linked moiety X motif 21, Nudix motif 21, Pre-mRNA cleavage factor Im 25 kDa subunit, NUDT21, CFIM25, CPSF25, CPSF5
Target/Specificity NUDT21 (AAH01403, 1 a.a. ~ 227 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
Dilution WB~~1:500~1000
Format Clear, colorless solution in phosphate buffered saline, pH 7.2 .
StorageStore at -20°C or lower. Aliquot to avoid repeated freezing and thawing.
PrecautionsNUDT21 Antibody (monoclonal) (M12) is for research use only and not for use in diagnostic or therapeutic procedures.
Citations (0)

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The protein encoded by this gene is one subunit of a cleavage factor required for 3' RNA cleavage and polyadenylation processing. The interaction of the protein with the RNA is one of the earliest steps in the assembly of the 3' end processing complex and facilitates the recruitment of other processing factors. This gene encodes the 25kD subunit of the protein complex, which is composed of four polypeptides.


Structural basis of UGUA recognition by the Nudix protein CFI(m)25 and implications for a regulatory role in mRNA 3' processing. Yang Q, et al. Proc Natl Acad Sci U S A, 2010 Jun 1. PMID 20479262.The crystal structure of human cleavage and polyadenylation specific factor-5 reveals a dimeric Nudix protein with a conserved catalytic site. Tr?saugues L, et al. Proteins, 2008 Dec. PMID 18767156.Crystal structure of the 25 kDa subunit of human cleavage factor Im. Coseno M, et al. Nucleic Acids Res, 2008 Jun. PMID 18445629.Large-scale mapping of human protein-protein interactions by mass spectrometry. Ewing RM, et al. Mol Syst Biol, 2007. PMID 17353931.Multiple histone deacetylases and the CREB-binding protein regulate pre-mRNA 3'-end processing. Shimazu T, et al. J Biol Chem, 2007 Feb 16. PMID 17172643.

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$ 315.00
Cat# AT3130a
(40 western blots)
Availability: 7-10 days
Bulk Size
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