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NUDT5 Antibody (monoclonal) (M04)

Mouse monoclonal antibody raised against a partial recombinant NUDT5.

     
  • WB - NUDT5 Antibody (monoclonal) (M04) AT3133a
    Antibody Reactive Against Recombinant Protein.Western Blot detection against Immunogen (36.74 KDa) .
    detail
  • WB - NUDT5 Antibody (monoclonal) (M04) AT3133a
    Western Blot analysis of NUDT5 expression in transfected 293T cell line by NUDT5 monoclonal antibody (M04), clone 2A3.

    Lane 1: NUDT5 transfected lysate(24.3 KDa).
    Lane 2: Non-transfected lysate.
    detail
  • WB - NUDT5 Antibody (monoclonal) (M04) AT3133a
    Western blot analysis of NUDT5 over-expressed 293 cell line, cotransfected with NUDT5 Validated Chimera RNAi ( (Cat # AT3133a )
    detail
  • E - NUDT5 Antibody (monoclonal) (M04) AT3133a
    Detection limit for recombinant GST tagged NUDT5 is approximately 1ng/ml as a capture antibody.
    detail
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
WB, E
Primary Accession Q9UKK9
Other Accession NM_014142
Reactivity Human
Host mouse
Clonality Monoclonal
Isotype IgG1 Kappa
Clone Names 2A3
Calculated MW 24328 Da
Additional Information
Gene ID 11164
Other Names ADP-sugar pyrophosphatase, 8-oxo-dGDP phosphatase, Nucleoside diphosphate-linked moiety X motif 5, Nudix motif 5, YSA1H, NUDT5
Target/Specificity NUDT5 (NP_054861, 120 a.a. ~ 219 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
Dilution WB~~1:500~1000
Format Clear, colorless solution in phosphate buffered saline, pH 7.2 .
StorageStore at -20°C or lower. Aliquot to avoid repeated freezing and thawing.
PrecautionsNUDT5 Antibody (monoclonal) (M04) is for research use only and not for use in diagnostic or therapeutic procedures.
Research Areas
Citations (0)
citation

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Background

Nudix hydrolases, such as NUDT5, eliminate toxic nucleotide derivatives from the cell and regulate the levels of important signaling nucleotides and their metabolites (McLennan, 1999 [PubMed 10373642]).

References

Suppression of mutagenesis by 8-hydroxy-2'-deoxyguanosine 5'-triphosphate (7,8-dihydro-8-oxo-2'-deoxyguanosine 5'-triphosphate) by human MTH1, MTH2, and NUDT5. Hori M, et al. Free Radic Biol Med, 2010 May 1. PMID 20144704.NUDT5 hydrolyzes oxidized deoxyribonucleoside diphosphates with broad substrate specificity. Kamiya H, et al. DNA Repair (Amst), 2009 Oct 2. PMID 19699693.Molecular mechanism of ADP-ribose hydrolysis by human NUDT5 from structural and kinetic studies. Zha M, et al. J Mol Biol, 2008 Jun 6. PMID 18462755.A human protein-protein interaction network: a resource for annotating the proteome. Stelzl U, et al. Cell, 2005 Sep 23. PMID 16169070.Immunoaffinity profiling of tyrosine phosphorylation in cancer cells. Rush J, et al. Nat Biotechnol, 2005 Jan. PMID 15592455.

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$ 350.00
Cat# AT3133a
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