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NUP133 Antibody (monoclonal) (M01)

Mouse monoclonal antibody raised against a partial recombinant NUP133.

     
  • WB - NUP133 Antibody (monoclonal) (M01) AT3135a
    Antibody Reactive Against Recombinant Protein.Western Blot detection against Immunogen (35.31 KDa) .
    detail
  • WB - NUP133 Antibody (monoclonal) (M01) AT3135a
    NUP133 monoclonal antibody (M01), clone 3E8 Western Blot analysis of NUP133 expression in HeLa ( (Cat # AT3135a )
    detail
  • IHC - NUP133 Antibody (monoclonal) (M01) AT3135a
    Immunoperoxidase of monoclonal antibody to NUP133 on formalin-fixed paraffin-embedded human stomach. [antibody concentration 3 ug/ml]
    detail
  • E - NUP133 Antibody (monoclonal) (M01) AT3135a
    Detection limit for recombinant GST tagged NUP133 is 0.03 ng/ml as a capture antibody.
    detail
  • SPECIFICATION
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
WB, IHC, E
Primary Accession Q8WUM0
Other Accession NM_018230
Reactivity Human
Host mouse
Clonality Monoclonal
Isotype IgG2a Kappa
Clone Names 3E9
Calculated MW 128979 Da
Additional Information
Gene ID 55746
Other Names Nuclear pore complex protein Nup133, 133 kDa nucleoporin, Nucleoporin Nup133, NUP133
Target/Specificity NUP133 (NP_060700, 1069 a.a. ~ 1155 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
Dilution WB~~1:500~1000
Format Clear, colorless solution in phosphate buffered saline, pH 7.2 .
StorageStore at -20°C or lower. Aliquot to avoid repeated freezing and thawing.
PrecautionsNUP133 Antibody (monoclonal) (M01) is for research use only and not for use in diagnostic or therapeutic procedures.
Research Areas
Citations (0)
citation

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Background

The nuclear envelope creates distinct nuclear and cytoplasmic compartments in eukaryotic cells. It consists of two concentric membranes perforated by nuclear pores, large protein complexes that form aqueous channels to regulate the flow of macromolecules between the nucleus and the cytoplasm. These complexes are composed of at least 100 different polypeptide subunits, many of which belong to the nucleoporin family. The nucleoporin protein encoded by this gene displays evolutionarily conserved interactions with other nucleoporins. This protein, which localizes to both sides of the nuclear pore complex at interphase, remains associated with the complex during mitosis and is targeted at early stages to the reforming nuclear envelope. This protein also localizes to kinetochores of mitotic cells.

References

1.Nuclear Distributions of NUP62 and NUP214 Suggest Architectural Diversity and Spatial Patterning among Nuclear Pore Complexes.Kinoshita Y, Kalir T, Dottino P, Kohtz DS.PLoS One. 2012;7(4):e36137. Epub 2012 Apr 27.2.Alterations in Nuclear Pore Architecture Allow Cancer Cell Entry into or Exit from Drug-Resistant Dormancy.Kinoshita Y, Kalir T, Rahaman J, Dottino P, Stave Kohtz D.Am J Pathol. 2011 Nov 7.

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$ 350.00
Cat# AT3135a
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Availability: 7-10 days
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