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>   home   >   Products   >   Primary Antibodies   >   Cell Biology   >   PCNA Antibody (monoclonal) (M02)   

PCNA Antibody (monoclonal) (M02)

Mouse monoclonal antibody raised against a full length recombinant PCNA.

  • WB - PCNA Antibody (monoclonal) (M02) AT3236a
    Antibody Reactive Against Recombinant Protein.Western Blot detection against Immunogen (54.45 KDa) .
  • WB - PCNA Antibody (monoclonal) (M02) AT3236a
    PCNA monoclonal antibody (M02), clone 1G7 Western Blot analysis of PCNA expression in Hela ( (Cat # AT3236a )
  • WB - PCNA Antibody (monoclonal) (M02) AT3236a
    Western Blot analysis of PCNA expression in transfected 293T cell line by PCNA monoclonal antibody (M02), clone 1G7.

    Lane 1: PCNA transfected lysate(28.8 KDa).
    Lane 2: Non-transfected lysate.
  • IF - PCNA Antibody (monoclonal) (M02) AT3236a
    Immunofluorescence of monoclonal antibody to PCNA on HeLa cell. [antibody concentration 10 ug/ml]
  • IP - PCNA Antibody (monoclonal) (M02) AT3236a
    Immunoprecipitation of PCNA transfected lysate using anti-PCNA monoclonal antibody and Protein A Magnetic Bead (U0007), and immunoblotted with PCNA MaxPab rabbit polyclonal antibody.
  • E - PCNA Antibody (monoclonal) (M02) AT3236a
    Detection limit for recombinant GST tagged PCNA is approximately 0.1ng/ml as a capture antibody.
Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary Accession P12004
Other Accession BC000491
Reactivity Human
Host mouse
Clonality Monoclonal
Isotype IgG2a kappa
Clone Names 1G7
Calculated MW 28769 Da
Additional Information
Gene ID 5111
Other Names Proliferating cell nuclear antigen, PCNA, Cyclin, PCNA
Target/Specificity PCNA (AAH00491, 1 a.a. ~ 261 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
Dilution WB~~1:500~1000
Format Clear, colorless solution in phosphate buffered saline, pH 7.2 .
StorageStore at -20°C or lower. Aliquot to avoid repeated freezing and thawing.
PrecautionsPCNA Antibody (monoclonal) (M02) is for research use only and not for use in diagnostic or therapeutic procedures.
Research Areas
Citations (0)

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The protein encoded by this gene is found in the nucleus and is a cofactor of DNA polymerase delta. The encoded protein acts as a homotrimer and helps increase the processivity of leading strand synthesis during DNA replication. In response to DNA damage, this protein is ubiquitinated and is involved in the RAD6-dependent DNA repair pathway. Two transcript variants encoding the same protein have been found for this gene. Pseudogenes of this gene have been described on chromosome 4 and on the X chromosome.


1.The RAD51-stimulatory compound RS-1 can exploit the RAD51 overexpression that exists in cancer cells and tumors.Mason JM, Logan HL, Budke B, Wu M, Pawlowski M, Weichselbaum RR, Kozikowski AP, Bishop DK, Connell PPCancer Res. 2014 Apr 21.

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$ 315.00
Cat# AT3236a
(40 western blots)
Availability: 7-10 days
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