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PCNA Antibody (monoclonal) (M04)

Mouse monoclonal antibody raised against a full length recombinant PCNA.

     
  • WB - PCNA Antibody (monoclonal) (M04) AT3237a
    Antibody Reactive Against Recombinant Protein.Western Blot detection against Immunogen (54.45 KDa) .
    detail
  • WB - PCNA Antibody (monoclonal) (M04) AT3237a
    PCNA monoclonal antibody (M04), clone S1. Western Blot analysis of PCNA expression in PC-12 ( (Cat # AT3237a )
    detail
  • WB - PCNA Antibody (monoclonal) (M04) AT3237a
    PCNA monoclonal antibody (M04), clone S1 Western Blot analysis of PCNA expression in Hela S3 NE ( (Cat # AT3237a )
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  • WB - PCNA Antibody (monoclonal) (M04) AT3237a
    PCNA monoclonal antibody (M04), clone S1. Western Blot analysis of PCNA expression in Raw 264.7 ( (Cat # AT3237a )
    detail
  • WB - PCNA Antibody (monoclonal) (M04) AT3237a
    PCNA monoclonal antibody (M04), clone S1. Western Blot analysis of PCNA expression in NIH/3T3 ( (Cat # AT3237a )
    detail
  • WB - PCNA Antibody (monoclonal) (M04) AT3237a
    Western Blot analysis of PCNA expression in transfected 293T cell line by PCNA monoclonal antibody (M04), clone S1.

    Lane 1: PCNA transfected lysate(28.8 KDa).
    Lane 2: Non-transfected lysate.
    detail
  • E - PCNA Antibody (monoclonal) (M04) AT3237a
    Detection limit for recombinant GST tagged PCNA is approximately 0.1ng/ml as a capture antibody.
    detail
  • SPECIFICATION
  • CITATIONS
  • PROTOCOLS
  • BACKGROUND
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
WB
Primary Accession P12004
Other Accession BC000491
Reactivity Human, Mouse, Rat
Host mouse
Clonality Monoclonal
Isotype IgG2b Kappa
Clone Names S1
Calculated MW 28769 Da
Additional Information
Gene ID 5111
Other Names Proliferating cell nuclear antigen, PCNA, Cyclin, PCNA
Target/Specificity PCNA (AAH00491, 1 a.a. ~ 261 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
Dilution WB~~1:500~1000
Format Clear, colorless solution in phosphate buffered saline, pH 7.2 .
StorageStore at -20°C or lower. Aliquot to avoid repeated freezing and thawing.
PrecautionsPCNA Antibody (monoclonal) (M04) is for research use only and not for use in diagnostic or therapeutic procedures.
Research Areas
Citations (0)
citation

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Background

The protein encoded by this gene is found in the nucleus and is a cofactor of DNA polymerase delta. The encoded protein acts as a homotrimer and helps increase the processivity of leading strand synthesis during DNA replication. In response to DNA damage, this protein is ubiquitinated and is involved in the RAD6-dependent DNA repair pathway. Two transcript variants encoding the same protein have been found for this gene. Pseudogenes of this gene have been described on chromosome 4 and on the X chromosome.

References

1.DNA Topoisomerase III Alpha Regulates p53-Mediated Tumor Suppression.Hsieh MY, Fan JR, Chang HW, Chen HC, Shen TL, Teng SC, Yeh YH, Li TKClin Cancer Res. 2014 Mar 15;20(6):1489-501. doi: 10.1158/1078-0432.CCR-13-1997. Epub 2014 Feb 13.

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$ 350.00
Cat# AT3237a
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Availability: 7-10 days
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