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PDHB Antibody (monoclonal) (M03)

Mouse monoclonal antibody raised against a partial recombinant PDHB.

  • WB - PDHB Antibody (monoclonal) (M03) AT3259a
    Antibody Reactive Against Recombinant Protein.Western Blot detection against Immunogen (37.84 KDa) .
  • WB - PDHB Antibody (monoclonal) (M03) AT3259a
    PDHB monoclonal antibody (M03), clone 2B2 Western Blot analysis of PDHB expression in HeLa ( (Cat # AT3259a )
  • WB - PDHB Antibody (monoclonal) (M03) AT3259a
    Western Blot analysis of PDHB expression in transfected 293T cell line by PDHB monoclonal antibody (M03), clone 2B2.

    Lane 1: PDHB transfected lysate(39.2 KDa).
    Lane 2: Non-transfected lysate.
  • IHC - PDHB Antibody (monoclonal) (M03) AT3259a
    Immunoperoxidase of monoclonal antibody to PDHB on formalin-fixed paraffin-embedded human heart. [antibody concentration 3 ug/ml]
  • E - PDHB Antibody (monoclonal) (M03) AT3259a
    Detection limit for recombinant GST tagged PDHB is approximately 1ng/ml as a capture antibody.
Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary Accession P11177
Other Accession NM_000925
Reactivity Human
Host mouse
Clonality Monoclonal
Isotype IgG1 Kappa
Clone Names 2B2
Calculated MW 39233 Da
Additional Information
Gene ID 5162
Other Names Pyruvate dehydrogenase E1 component subunit beta, mitochondrial, PDHE1-B, PDHB, PHE1B
Target/Specificity PDHB (NP_000916, 250 a.a. ~ 359 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
Dilution WB~~1:500~1000
Format Clear, colorless solution in phosphate buffered saline, pH 7.2 .
StorageStore at -20°C or lower. Aliquot to avoid repeated freezing and thawing.
PrecautionsPDHB Antibody (monoclonal) (M03) is for research use only and not for use in diagnostic or therapeutic procedures.
Research Areas
Citations (0)

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The pyruvate dehydrogenase (PDH) complex is a nuclear-encoded mitochondrial multienzyme complex that catalyzes the overall conversion of pyruvate to acetyl-CoA and CO(2), and provides the primary link between glycolysis and the tricarboxylic acid (TCA) cycle. The PDH complex is composed of multiple copies of three enzymatic components: pyruvate dehydrogenase (E1), dihydrolipoamide acetyltransferase (E2) and lipoamide dehydrogenase (E3). The E1 enzyme is a heterotetramer of two alpha and two beta subunits. This gene encodes the E1 beta subunit. Mutations in this gene are associated with pyruvate dehydrogenase E1-beta deficiency. Alternatively spliced transcript variants have been found for this gene.


1.Proteomics of uveal melanomas suggests HSP-27 as a possible surrogate marker of chromosome 3 loss.Coupland SE, Vorum H, Mandal N, Kalirai H, Honore B, Urbak SF, Lake SL, Dopierala J, Damato B.Invest Ophthalmol Vis Sci. 2010 Jan;51(1):12-20. Epub 2009 Jul 30.

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$ 315.00
Cat# AT3259a
(40 western blots)
Availability: 7-10 days
Bulk Size
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