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PGGT1B Antibody (monoclonal) (M02)

Mouse monoclonal antibody raised against a partial recombinant PGGT1B.

     
  • IF - PGGT1B Antibody (monoclonal) (M02) AT3281a
    Immunofluorescence of monoclonal antibody to PGGT1B on HeLa cell. [antibody concentration 10 ug/ml]
    detail
  • WB - PGGT1B Antibody (monoclonal) (M02) AT3281a
    Antibody Reactive Against Recombinant Protein.Western Blot detection against Immunogen (37.29 KDa) .
    detail
  • WB - PGGT1B Antibody (monoclonal) (M02) AT3281a
    PGGT1B monoclonal antibody (M02), clone 5E4 Western Blot analysis of PGGT1B expression in HeLa ( (Cat # AT3281a )
    detail
  • WB - PGGT1B Antibody (monoclonal) (M02) AT3281a
    PGGT1B monoclonal antibody (M02), clone 5E4. Western Blot analysis of PGGT1B expression in PC-12 ( (Cat # AT3281a )
    detail
  • IHC - PGGT1B Antibody (monoclonal) (M02) AT3281a
    Immunoperoxidase of monoclonal antibody to PGGT1B on formalin-fixed paraffin-embedded human kidney. [antibody concentration 1.2 ug/ml]
    detail
  • E - PGGT1B Antibody (monoclonal) (M02) AT3281a
    Detection limit for recombinant GST tagged PGGT1B is approximately 1ng/ml as a capture antibody.
    detail
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
WB, IHC, IF, E
Primary Accession P53609
Other Accession NM_005023
Reactivity Human, Rat
Host mouse
Clonality Monoclonal
Isotype IgG2a Kappa
Clone Names 5E5
Calculated MW 42368 Da
Additional Information
Gene ID 5229
Other Names Geranylgeranyl transferase type-1 subunit beta, Geranylgeranyl transferase type I subunit beta, GGTase-I-beta, Type I protein geranyl-geranyltransferase subunit beta, PGGT1B
Target/Specificity PGGT1B (NP_005014, 1 a.a. ~ 105 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
Dilution WB~~1:500~1000
Format Clear, colorless solution in phosphate buffered saline, pH 7.2 .
StorageStore at -20°C or lower. Aliquot to avoid repeated freezing and thawing.
PrecautionsPGGT1B Antibody (monoclonal) (M02) is for research use only and not for use in diagnostic or therapeutic procedures.
Research Areas
Citations (0)
citation

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Background

Protein geranylgeranyltransferase type I (GGTase-I) transfers a geranylgeranyl group to the cysteine residue of candidate proteins containing a C-terminal CAAX motif in which 'A' is an aliphatic amino acid and 'X' is leucine (summarized by Zhang et al., 1994 [PubMed 8106351]). The enzyme is composed of a 48-kD alpha subunit (FNTA; MIM 134635) and a 43-kD beta subunit, encoded by the PGGT1B gene. The FNTA gene encodes the alpha subunit for both GGTase-I and the related enzyme farnesyltransferase.

References

1.Genetic studies on the functional relevance of the protein prenyltransferases in skin keratinocytes.Lee R, Chang SY, Trinh H, Tu Y, White AC, Davies BS, Bergo MO, Fong LG, Lowry WE, Young SG.Hum Mol Genet. 2010 Feb 11. [Epub ahead of print]

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$ 350.00
Cat# AT3281a
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