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PGM1 Antibody (monoclonal) (M01)

Mouse monoclonal antibody raised against a full length recombinant PGM1.

     
  • IF - PGM1 Antibody (monoclonal) (M01) AT3282a
    Immunofluorescence of monoclonal antibody to PGM1 on HeLa cell. [antibody concentration 20 ug/ml]
    detail
  • IP - PGM1 Antibody (monoclonal) (M01) AT3282a
    Immunoprecipitation of PGM1 transfected lysate using anti-PGM1 monoclonal antibody and Protein A Magnetic Bead (U0007), and immunoblotted with PGM1 MaxPab rabbit polyclonal antibody.
    detail
  • WB - PGM1 Antibody (monoclonal) (M01) AT3282a
    Antibody Reactive Against Recombinant Protein.Western Blot detection against Immunogen (87.56 KDa) .
    detail
  • WB - PGM1 Antibody (monoclonal) (M01) AT3282a
    PGM1 monoclonal antibody (M01), clone 3B8-H4 Western Blot analysis of PGM1 expression in Jurkat ( (Cat # AT3282a )
    detail
  • WB - PGM1 Antibody (monoclonal) (M01) AT3282a
    Western Blot analysis of PGM1 expression in transfected 293T cell line by PGM1 monoclonal antibody (M01), clone 3B8-H4.

    Lane 1: PGM1 transfected lysate(61.4 KDa).
    Lane 2: Non-transfected lysate.
    detail
  • WB - PGM1 Antibody (monoclonal) (M01) AT3282a
    Western blot analysis of PGM1 over-expressed 293 cell line, cotransfected with PGM1 Validated Chimera RNAi ( (Cat # AT3282a )
    detail
  • E - PGM1 Antibody (monoclonal) (M01) AT3282a
    Detection limit for recombinant GST tagged PGM1 is 0.3 ng/ml as a capture antibody.
    detail
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
WB, IF, IP, E
Primary Accession P36871
Other Accession BC019920
Reactivity Human
Host mouse
Clonality Monoclonal
Isotype IgG1 kappa
Clone Names 3B8-H4
Calculated MW 61449 Da
Additional Information
Gene ID 5236
Other Names Phosphoglucomutase-1, PGM 1, Glucose phosphomutase 1, PGM1
Target/Specificity PGM1 (AAH19920, 1 a.a. ~ 562 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
Dilution WB~~1:500~1000
Format Clear, colorless solution in phosphate buffered saline, pH 7.2 .
StorageStore at -20°C or lower. Aliquot to avoid repeated freezing and thawing.
PrecautionsPGM1 Antibody (monoclonal) (M01) is for research use only and not for use in diagnostic or therapeutic procedures.
Research Areas
Citations (0)
citation

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Background

The protein encoded by this gene is an isozyme of phosphoglucomutase (PGM) and belongs to the phosphohexose mutase family. There are several PGM isozymes, which are encoded by different genes and catalyze the transfer of phosphate between the 1 and 6 positions of glucose. In most cell types, this PGM isozyme is predominant, representing about 90% of total PGM activity. In red cells, PGM2 is a major isozyme. This gene is highly polymorphic. Mutations in this gene cause glycogen storage disease type 14. Alternativley spliced transcript variants encoding different isoforms have been identified in this gene.

References

1.Functional analysis of beef tenderness.Guillemin N, Bonnet M, Jurie C, Picard B.J Proteomics. 2011 Aug 9. [Epub ahead of print]

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$ 350.00
Cat# AT3282a
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