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PHB Antibody (monoclonal) (M01)

Mouse monoclonal antibody raised against a full length recombinant PHB.

  • WB - PHB Antibody (monoclonal) (M01) AT3292a
    Antibody Reactive Against Recombinant Protein.Western Blot detection against Immunogen (55.66 KDa) .
  • WB - PHB Antibody (monoclonal) (M01) AT3292a
    PHB monoclonal antibody (M01), clone 3F4-2B2 Western Blot analysis of PHB expression in Hela ( (Cat # AT3292a )
  • IHC - PHB Antibody (monoclonal) (M01) AT3292a
    Immunoperoxidase of monoclonal antibody to PHB on formalin-fixed paraffin-embedded human colon adenocarcinoma. [antibody concentration 5 ug/ml]
  • IHC - PHB Antibody (monoclonal) (M01) AT3292a
    Immunoperoxidase of monoclonal antibody to PHB on formalin-fixed paraffin-embedded human tonsil tissue.[antibody concentration 5 ug/ml]
  • IF - PHB Antibody (monoclonal) (M01) AT3292a
    Immunofluorescence of monoclonal antibody to PHB on HeLa cell. [antibody concentration 1 ~ 10 ug/ml]
  • E - PHB Antibody (monoclonal) (M01) AT3292a
    Detection limit for recombinant GST tagged PHB is approximately 0.03ng/ml as a capture antibody.
Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary Accession P35232
Other Accession BC013401
Reactivity Human
Host mouse
Clonality Monoclonal
Isotype IgG2a kappa
Clone Names 3F4-2B2
Calculated MW 29804 Da
Additional Information
Gene ID 5245
Other Names Prohibitin, PHB
Target/Specificity PHB (AAH13401, 1 a.a. ~ 272 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
Dilution WB~~1:500~1000
Format Clear, colorless solution in phosphate buffered saline, pH 7.2 .
StorageStore at -20°C or lower. Aliquot to avoid repeated freezing and thawing.
PrecautionsPHB Antibody (monoclonal) (M01) is for research use only and not for use in diagnostic or therapeutic procedures.
Research Areas
Citations (0)

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Prohibitin is an evolutionarily conserved gene that is ubiquitously expressed. It is thought to be a negative regulator of cell proliferation and may be a tumor suppressor. Mutations in PHB have been linked to sporadic breast cancer. Prohibitin is expressed as two transcripts with varying lengths of 3' untranslated region. The longer transcript is present at higher levels in proliferating tissues and cells, suggesting that this longer 3' untranslated region may function as a trans-acting regulatory RNA.


1.Gonad differential proteins revealed with proteomics in oyster (Saccostrea cucullata) using alga as food contaminated with cadmium.Zhu B, Gao KS, Wang KJ, Ke CH, Huang HQ.Chemosphere. 2012 Jan 7. [Epub ahead of print]

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$ 315.00
Cat# AT3292a
(40 western blots)
Availability: 7-10 days
Bulk Size
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