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PHC1 Antibody (monoclonal) (M05)

Mouse monoclonal antibody raised against a partial recombinant PHC1.

     
  • WB - PHC1 Antibody (monoclonal) (M05) AT3293a
    Antibody Reactive Against Recombinant Protein.Western Blot detection against Immunogen (37.11 KDa) .
    detail
  • WB - PHC1 Antibody (monoclonal) (M05) AT3293a
    PHC1 monoclonal antibody (M05), clone 3G1. Western Blot analysis of PHC1 expression in MCF-7.
    detail
  • E - PHC1 Antibody (monoclonal) (M05) AT3293a
    Detection limit for recombinant GST tagged PHC1 is approximately 3ng/ml as a capture antibody.
    detail
  • SPECIFICATION
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  • BACKGROUND
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
WB, E
Primary Accession P78364
Other Accession NM_004426
Reactivity Human
Host mouse
Clonality Monoclonal
Isotype IgG2b Kappa
Clone Names 3G1
Calculated MW 105534 Da
Additional Information
Gene ID 1911
Other Names Polyhomeotic-like protein 1, hPH1, Early development regulatory protein 1, PHC1, EDR1, PH1
Target/Specificity PHC1 (NP_004417, 751 a.a. ~ 851 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
Dilution WB~~1:500~1000
Format Clear, colorless solution in phosphate buffered saline, pH 7.2 .
StorageStore at -20°C or lower. Aliquot to avoid repeated freezing and thawing.
PrecautionsPHC1 Antibody (monoclonal) (M05) is for research use only and not for use in diagnostic or therapeutic procedures.
Research Areas
Citations (0)
citation

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Background

This gene is a homolog of the Drosophila polyhomeotic gene, which is a member of the Polycomb group of genes. The gene product is a component of a multimeric protein complex that contains EDR2 and the vertebrate Polycomb protein BMH1. The gene product, the EDR2 protein, and the Drosophila polyhomeotic protein share 2 highly conserved domains, named homology domains I and II. These domains are involved in protein-protein interactions and may mediate heterodimerization of the protein encoded by this gene and the EDR2 protein.

References

Polycomb-group complex 1 acts as an E3 ubiquitin ligase for Geminin to sustain hematopoietic stem cell activity. Ohtsubo M, et al. Proc Natl Acad Sci U S A, 2008 Jul 29. PMID 18650381.Toward a confocal subcellular atlas of the human proteome. Barbe L, et al. Mol Cell Proteomics, 2008 Mar. PMID 18029348.Towards a proteome-scale map of the human protein-protein interaction network. Rual JF, et al. Nature, 2005 Oct 20. PMID 16189514.A human protein-protein interaction network: a resource for annotating the proteome. Stelzl U, et al. Cell, 2005 Sep 23. PMID 16169070.MAPKAP kinase 3pK phosphorylates and regulates chromatin association of the polycomb group protein Bmi1. Voncken JW, et al. J Biol Chem, 2005 Feb 18. PMID 15563468.

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$ 350.00
Cat# AT3293a
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