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PKNOX2 Antibody (monoclonal) (M01)

Mouse monoclonal antibody raised against a partial recombinant PKNOX2.

     
  • IF - PKNOX2 Antibody (monoclonal) (M01) AT3323a
    Immunofluorescence of monoclonal antibody to PKNOX2 on HeLa cell. [antibody concentration 10 ug/ml]
    detail
  • WB - PKNOX2 Antibody (monoclonal) (M01) AT3323a
    Antibody Reactive Against Recombinant Protein.Western Blot detection against Immunogen (36.74 KDa) .
    detail
  • WB - PKNOX2 Antibody (monoclonal) (M01) AT3323a
    PKNOX2 monoclonal antibody (M01), clone 4B6 Western Blot analysis of PKNOX2 expression in Hela S3 NE ( (Cat # AT3323a )
    detail
  • WB - PKNOX2 Antibody (monoclonal) (M01) AT3323a
    PKNOX2 monoclonal antibody (M01), clone 4B6. Western Blot analysis of PKNOX2 expression in NIH/3T3 ( (Cat # AT3323a )
    detail
  • WB - PKNOX2 Antibody (monoclonal) (M01) AT3323a
    Western Blot analysis of PKNOX2 expression in transfected 293T cell line by PKNOX2 monoclonal antibody (M01), clone 4B6.

    Lane 1: PKNOX2 transfected lysate(51.9 KDa).
    Lane 2: Non-transfected lysate.
    detail
  • WB - PKNOX2 Antibody (monoclonal) (M01) AT3323a
    Western blot analysis of PKNOX2 over-expressed 293 cell line, cotransfected with PKNOX2 Validated Chimera RNAi ( (Cat # AT3323a )
    detail
  • E - PKNOX2 Antibody (monoclonal) (M01) AT3323a
    Detection limit for recombinant GST tagged PKNOX2 is 0.03 ng/ml as a capture antibody.
    detail
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
WB, IF, E
Primary Accession Q96KN3
Other Accession NM_022062
Reactivity Human, Mouse
Host mouse
Clonality Monoclonal
Isotype IgG2b Kappa
Clone Names 4B6
Calculated MW 52028 Da
Additional Information
Other Names Homeobox protein PKNOX2, Homeobox protein PREP-2, PBX/knotted homeobox 2, PKNOX2, PREP2
Target/Specificity PKNOX2 (NP_071345, 116 a.a. ~ 215 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
Dilution WB~~1:500~1000
Format Clear, colorless solution in phosphate buffered saline, pH 7.2 .
StorageStore at -20°C or lower. Aliquot to avoid repeated freezing and thawing.
PrecautionsPKNOX2 Antibody (monoclonal) (M01) is for research use only and not for use in diagnostic or therapeutic procedures.
Citations (0)
citation

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Background

Homeodomain proteins are sequence-specific transcription factors that share a highly conserved DNA-binding domain and play fundamental roles in cell proliferation, differentiation, and death. PKNOX2 belongs to the TALE (3-amino acid loop extension) class of homeodomain proteins characterized by a 3-amino acid extension between alpha helices 1 and 2 within the homeodomain (Imoto et al., 2001 [PubMed 11549286]).

References

Variation at the NFATC2 Locus Increases the Risk of Thiazolinedinedione-Induced Edema in the Diabetes REduction Assessment with ramipril and rosiglitazone Medication (DREAM) Study. Bailey SD, et al. Diabetes Care, 2010 Jul 13. PMID 20628086.Personalized smoking cessation: interactions between nicotine dose, dependence and quit-success genotype score. Rose JE, et al. Mol Med, 2010 Jul-Aug. PMID 20379614.Gene-centric association signals for lipids and apolipoproteins identified via the HumanCVD BeadChip. Talmud PJ, et al. Am J Hum Genet, 2009 Nov. PMID 19913121.PKNOX2 gene is significantly associated with substance dependence in European-origin women. Chen X, et al. Proc Natl Acad Sci U S A, 2009 Sep 10. PMID 19721000.Diversification of transcriptional modulation: large-scale identification and characterization of putative alternative promoters of human genes. Kimura K, et al. Genome Res, 2006 Jan. PMID 16344560.

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$ 350.00
Cat# AT3323a
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Availability: 7-10 days
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