Register or Login
  • All
  • Uniprot Id
  • Catalog #
  • Peptide Sequence
>   home   >   Products   >   Primary Antibodies   >   Signal Transduction   >   PML Antibody (monoclonal) (M02)   

PML Antibody (monoclonal) (M02)

Mouse monoclonal antibody raised against a partial recombinant PML.

  • WB - PML Antibody (monoclonal) (M02) AT3354a
    Antibody Reactive Against Recombinant Protein.Western Blot detection against Immunogen (36.63 KDa) .
  • WB - PML Antibody (monoclonal) (M02) AT3354a
    PML monoclonal antibody (M02), clone 1D12. Western Blot analysis of PML expression in PC-12((Cat # AT3354a )
  • WB - PML Antibody (monoclonal) (M02) AT3354a
    PML monoclonal antibody (M02), clone 1D12 Western Blot analysis of PML expression in Hela S3 NE ( (Cat # AT3354a )
  • IF - PML Antibody (monoclonal) (M02) AT3354a
    Immunofluorescence of monoclonal antibody to PML on HeLa cell. [antibody concentration 10 ug/ml]
  • E - PML Antibody (monoclonal) (M02) AT3354a
    Detection limit for recombinant GST tagged PML is approximately 0.3ng/ml as a capture antibody.
  •  - PML Antibody (monoclonal) (M02) AT3354a
    Proximity Ligation Analysis of protein-protein interactions between TP53 and PML HeLa cells were stained with anti-TP53 rabbit purified polyclonal 1:1200 and anti-PML mouse monoclonal antibody 1:50. Each red dot represents the detection of protein-protein interaction complex, and nuclei were counterstained with DAPI (blue).
Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary Accession P29590
Other Accession BC000080
Reactivity Human, Rat
Host mouse
Clonality Monoclonal
Isotype IgG2a Kappa
Clone Names 1D12
Calculated MW 97551 Da
Additional Information
Gene ID 5371
Other Names Protein PML, Promyelocytic leukemia protein, RING finger protein 71, Tripartite motif-containing protein 19, PML, MYL, PP8675, RNF71, TRIM19
Target/Specificity PML (AAH00080, 411 a.a. ~ 510 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
Dilution WB~~1:500~1000
Format Clear, colorless solution in phosphate buffered saline, pH 7.2 .
StorageStore at -20°C or lower. Aliquot to avoid repeated freezing and thawing.
PrecautionsPML Antibody (monoclonal) (M02) is for research use only and not for use in diagnostic or therapeutic procedures.
Research Areas
Citations (0)

Thousands of laboratories across the world have published research that depended on the performance of antibodies from Abgent to advance their research. Check out links to articles that cite our products in major peer-reviewed journals, organized by research category.

Submit your citation using an Abgent antibody to, and receive a free "I Love Antibodies" mug.


The protein encoded by this gene is a member of the tripartite motif (TRIM) family. The TRIM motif includes three zinc-binding domains, a RING, a B-box type 1 and a B-box type 2, and a coiled-coil region. This phosphoprotein localizes to nuclear bodies where it functions as a transcription factor and tumor suppressor. Its expression is cell-cycle related and it regulates the p53 response to oncogenic signals. The gene is often involved in the translocation with the retinoic acid receptor alpha gene associated with acute promyelocytic leukemia (APL). Extensive alternative splicing of this gene results in several variations of the protein's central and C-terminal regions; all variants encode the same N-terminus. Alternatively spliced transcript variants encoding different isoforms have been identified.


PML down-regulation in soft tissue sarcomas. Vincenzi B, et al. J Cell Physiol, 2010 Sep. PMID 20578240.Analysis of t(15;17) chromosomal breakpoint sequences in therapy-related versus de novo acute promyelocytic leukemia: association of DNA breaks with specific DNA motifs at PML and RARA loci. Hasan SK, et al. Genes Chromosomes Cancer, 2010 Aug. PMID 20544846.Two-step colocalization of MORC3 with PML nuclear bodies. Mimura Y, et al. J Cell Sci, 2010 Jun 15. PMID 20501696.Personalized smoking cessation: interactions between nicotine dose, dependence and quit-success genotype score. Rose JE, et al. Mol Med, 2010 Jul-Aug. PMID 20379614.Dysregulation of promyelocytic leukemia (PML) protein expression in preeclamptic placentae. Leavenworth JD, et al. Reprod Sci, 2010 Apr. PMID 20228380.

Abgent welcomes feedback from its customers.

If you have used an Abgent product and would like to share how it has performed, please click on the "Submit Review" button and provide the requested information. Our staff will examine and post your review and contact you if needed.

If you have any additional inquiries please email technical services at

$ 315.00
Cat# AT3354a
(40 western blots)
Availability: 7-10 days
Bulk Size
Seasonal Special on Bulk Order
Request Quote Here

Ordering Information

United States
AlbaniaAustraliaAustriaBelgiumBosnia & HerzegovinaBrazilBulgariaCanadaChinaCroatiaCyprusCzech RepublicDenmarkEstoniaFinlandFranceGermanyGreeceHong KongHungaryIcelandIndiaIndonesiaIrelandIsraelItalyJapanKoreaLatviaLithuaniaLuxembourgMacedoniaMalaysiaMaltaNetherlandsNew ZealandNorwayPakistanPolandPortugalRomaniaSerbiaSingaporeSlovakiaSloveniaSouth AfricaSpainSwedenSwitzerlandTaiwanTurkeyUnited KingdomUnited StatesVietnamOthers
Abgent, Inc.
(888) 735-7227 / (858) 622-0099
(858) 622-0609

Shipping Information

Domestic orders (in stock items)
Shipped out the same day. Orders placed after 1 PM (PST) will ship out the next business day.
International orders
Contact your local distributors
Santa Cruz Alternative
Terms & Conditions