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POLR2I Antibody (monoclonal) (M01)

Mouse monoclonal antibody raised against a partial recombinant POLR2I.

     
  • WB - POLR2I Antibody (monoclonal) (M01) AT3377a
    Antibody Reactive Against Recombinant Protein.Western Blot detection against Immunogen (36.74 KDa) .
    detail
  • E - POLR2I Antibody (monoclonal) (M01) AT3377a
    Detection limit for recombinant GST tagged POLR2I is approximately 0.03ng/ml as a capture antibody.
    detail
  • SPECIFICATION
  • CITATIONS
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
WB, E
Primary Accession P36954
Other Accession BC017112
Reactivity Human
Host mouse
Clonality Monoclonal
Isotype IgG1 Kappa
Clone Names 2B3
Calculated MW 14523 Da
Additional Information
Gene ID 5438
Other Names DNA-directed RNA polymerase II subunit RPB9, RNA polymerase II subunit B9, DNA-directed RNA polymerase II subunit I, RNA polymerase II 145 kDa subunit, RPB145, POLR2I
Target/Specificity POLR2I (AAH17112, 26 a.a. ~ 125 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
Dilution WB~~1:500~1000
Format Clear, colorless solution in phosphate buffered saline, pH 7.2 .
StorageStore at -20°C or lower. Aliquot to avoid repeated freezing and thawing.
PrecautionsPOLR2I Antibody (monoclonal) (M01) is for research use only and not for use in diagnostic or therapeutic procedures.
Citations (0)
citation

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Background

This gene encodes a subunit of RNA polymerase II, the polymerase responsible for synthesizing messenger RNA in eukaryotes. This subunit, in combination with two other polymerase subunits, forms the DNA binding domain of the polymerase, a groove in which the DNA template is transcribed into RNA. The product of this gene has two zinc finger motifs with conserved cysteines and the subunit does possess zinc binding activity.

References

Defining the human deubiquitinating enzyme interaction landscape. Sowa ME, et al. Cell, 2009 Jul 23. PMID 19615732.Will diverse Tat interactions lead to novel antiretroviral drug targets? Harrich D, et al. Curr Drug Targets, 2006 Dec. PMID 17168834.The regulation of HIV-1 transcription: molecular targets for chemotherapeutic intervention. Stevens M, et al. Med Res Rev, 2006 Sep. PMID 16838299.Inhibition of HIV-1 virus replication using small soluble Tat peptides. Agbottah E, et al. Virology, 2006 Feb 20. PMID 16289656.The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC). Gerhard DS, et al. Genome Res, 2004 Oct. PMID 15489334.

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$ 350.00
Cat# AT3377a
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Availability: 7-10 days
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