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PRG4 Antibody (monoclonal) (M01)

Mouse monoclonal antibody raised against a partial recombinant PRG4.

     
  • IF - PRG4 Antibody (monoclonal) (M01) AT3425a
    Immunofluorescence of monoclonal antibody to PRG4 on HeLa cell . [antibody concentration 10 ug/ml]
  • E - PRG4 Antibody (monoclonal) (M01) AT3425a
    Detection limit for recombinant GST tagged PRG4 is approximately 3ng/ml as a capture antibody.
  • SPECIFICATION
  • CITATIONS
  • PROTOCOLS
  • BACKGROUND
Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
IF, E
Primary Accession Q92954
Other Accession NM_005807
Reactivity Human
Host mouse
Clonality Monoclonal
Isotype IgG2a Kappa
Clone Names 2A6
Calculated MW 151077 Da
Additional Information
Gene ID 10216
Other Names Proteoglycan 4, Lubricin, Megakaryocyte-stimulating factor, Superficial zone proteoglycan, Proteoglycan 4 C-terminal part, PRG4, MSF, SZP
Target/Specificity PRG4 (NP_005798, 1305 a.a. ~ 1404 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
Format Clear, colorless solution in phosphate buffered saline, pH 7.2 .
StorageStore at -20°C or lower. Aliquot to avoid repeated freezing and thawing.
PrecautionsPRG4 Antibody (monoclonal) (M01) is for research use only and not for use in diagnostic or therapeutic procedures.
Research Areas
Citations (0)

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Background

The protein encoded by this gene is a large proteoglycan specifically synthesized by chondrocytes located at the surface of articular cartilage, and also by some synovial lining cells. This protein contains both chondroitin sulfate and keratan sulfate glycosaminoglycans. It functions as a boundary lubricant at the cartilage surface and contributes to the elastic absorption and energy dissipation of synovial fluid. Mutations in this gene result in camptodactyly-arthropathy-coxa vara-pericarditis syndrome. Multiple transcript variants encoding different isoforms have been found for this gene.

References

1.Interactive cytokine regulation of synoviocyte lubricant secretion.Blewis ME, Lao BJ, Schumacher BL, Bugbee WD, Sah RL, Firestein GS.Tissue Eng Part A. 2010 Apr;16(4):1329-37.

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$ 315.00
Cat# AT3425a
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