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PSMA1 Antibody (monoclonal) (M01)

Mouse monoclonal antibody raised against a full length recombinant PSMA1.

     
  • IF - PSMA1 Antibody (monoclonal) (M01) AT3453a
    Immunofluorescence of monoclonal antibody to PSMA1 on HeLa cell. [antibody concentration 60 ug/ml]
    detail
  • WB - PSMA1 Antibody (monoclonal) (M01) AT3453a
    Antibody Reactive Against Recombinant Protein.Western Blot detection against Immunogen (54.67 KDa) .
    detail
  • WB - PSMA1 Antibody (monoclonal) (M01) AT3453a
    PSMA1 monoclonal antibody (M01), clone 1D9-1C7 Western Blot analysis of PSMA1 expression in HeLa ( (Cat # AT3453a )
    detail
  • WB - PSMA1 Antibody (monoclonal) (M01) AT3453a
    Western Blot analysis of PSMA1 expression in transfected 293T cell line by PSMA1 monoclonal antibody (M01), clone 1D9-1C7.

    Lane 1: PSMA1 transfected lysate(30.2 KDa).
    Lane 2: Non-transfected lysate.
    detail
  • E - PSMA1 Antibody (monoclonal) (M01) AT3453a
    Detection limit for recombinant GST tagged PSMA1 is approximately 3ng/ml as a capture antibody.
    detail
  • SPECIFICATION
  • CITATIONS
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  • BACKGROUND
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
WB, IF
Primary Accession P25786
Other Accession BC002577
Reactivity Human
Host mouse
Clonality Monoclonal
Isotype IgG1 kappa
Clone Names 1D9-1C7
Calculated MW 29556 Da
Additional Information
Gene ID 5682
Other Names Proteasome subunit alpha type-1, 30 kDa prosomal protein, PROS-30, Macropain subunit C2, Multicatalytic endopeptidase complex subunit C2, Proteasome component C2, Proteasome nu chain, PSMA1, HC2, NU, PROS30, PSC2
Target/Specificity PSMA1 (AAH02577, 1 a.a. ~ 263 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
Dilution WB~~1:500~1000
Format Clear, colorless solution in phosphate buffered saline, pH 7.2 .
StorageStore at -20°C or lower. Aliquot to avoid repeated freezing and thawing.
PrecautionsPSMA1 Antibody (monoclonal) (M01) is for research use only and not for use in diagnostic or therapeutic procedures.
Research Areas
Citations (0)
citation

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Background

The proteasome is a multicatalytic proteinase complex with a highly ordered ring-shaped 20S core structure. The core structure is composed of 4 rings of 28 non-identical subunits; 2 rings are composed of 7 alpha subunits and 2 rings are composed of 7 beta subunits. Proteasomes are distributed throughout eukaryotic cells at a high concentration and cleave peptides in an ATP/ubiquitin-dependent process in a non-lysosomal pathway. An essential function of a modified proteasome, the immunoproteasome, is the processing of class I MHC peptides. This gene encodes a member of the peptidase T1A family, that is a 20S core alpha subunit. Alternative splicing results in multiple transcript variants encoding distinct isoforms.

References

1.The Role of Translationally Controlled Tumor Protein in Tumor Growth and Metastasis of Colon Adenocarcinoma Cells.Ma Q, Geng Y, Xu W, Wu Y, He F, Shu W, Huang M, Du H, Li M.J Proteome Res. 2009 Aug 11. [Epub ahead of print]

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$ 350.00
Cat# AT3453a
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