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>   home   >   Products   >   Primary Antibodies   >   Cell Biology   >   PSMB8 Antibody (monoclonal) (M01)   

PSMB8 Antibody (monoclonal) (M01)

Mouse monoclonal antibody raised against a partial recombinant PSMB8.

  • WB - PSMB8 Antibody (monoclonal) (M01) AT3463a
    Antibody Reactive Against Recombinant Protein.Western Blot detection against Immunogen (36.74 KDa) .
  • WB - PSMB8 Antibody (monoclonal) (M01) AT3463a
    Western Blot analysis of PSMB8 expression in transfected 293T cell line by PSMB8 monoclonal antibody (M01), clone 1B3.

    Lane 1: PSMB8 transfected lysate(29.8 KDa).
    Lane 2: Non-transfected lysate.
  • IHC - PSMB8 Antibody (monoclonal) (M01) AT3463a
    Immunoperoxidase of monoclonal antibody to PSMB8 on formalin-fixed paraffin-embedded human colon. [antibody concentration 3 ug/ml]
  • IF - PSMB8 Antibody (monoclonal) (M01) AT3463a
    Immunofluorescence of monoclonal antibody to PSMB8 on HeLa cell. [antibody concentration 10 ug/ml]
  • IP - PSMB8 Antibody (monoclonal) (M01) AT3463a
    Immunoprecipitation of PSMB8 transfected lysate using anti-PSMB8 monoclonal antibody and Protein A Magnetic Bead (U0007), and immunoblotted with PSMB8 MaxPab rabbit polyclonal antibody.
  • E - PSMB8 Antibody (monoclonal) (M01) AT3463a
    Detection limit for recombinant GST tagged PSMB8 is approximately 1ng/ml as a capture antibody.
Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary Accession P28062
Other Accession BC001114
Reactivity Human
Host Mouse
Clonality Monoclonal
Isotype IgG2a Kappa
Clone Names 1B3
Calculated MW 30354 Da
Additional Information
Gene ID 5696
Other Names Proteasome subunit beta type-8, Low molecular mass protein 7, Macropain subunit C13, Multicatalytic endopeptidase complex subunit C13, Proteasome component C13, Proteasome subunit beta-5i, Really interesting new gene 10 protein, PSMB8, LMP7, PSMB5i, RING10, Y2
Target/Specificity PSMB8 (AAH01114, 173 a.a. ~ 272 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
Dilution WB~~1:500~1000
Format Clear, colorless solution in phosphate buffered saline, pH 7.2 .
StorageStore at -20°C or lower. Aliquot to avoid repeated freezing and thawing.
PrecautionsPSMB8 Antibody (monoclonal) (M01) is for research use only and not for use in diagnostic or therapeutic procedures.
Research Areas
Citations (0)

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The proteasome is a multicatalytic proteinase complex with a highly ordered ring-shaped 20S core structure. The core structure is composed of 4 rings of 28 non-identical subunits; 2 rings are composed of 7 alpha subunits and 2 rings are composed of 7 beta subunits. Proteasomes are distributed throughout eukaryotic cells at a high concentration and cleave peptides in an ATP/ubiquitin-dependent process in a non-lysosomal pathway. An essential function of a modified proteasome, the immunoproteasome, is the processing of class I MHC peptides. This gene encodes a member of the proteasome B-type family, also known as the T1B family, that is a 20S core beta subunit. This gene is located in the class II region of the MHC (major histocompatibility complex). Expression of this gene is induced by gamma interferon and this gene product replaces catalytic subunit 3 (proteasome beta 5 subunit) in the immunoproteasome. Proteolytic processing is required to generate a mature subunit. Two alternative transcripts encoding two isoforms have been identified; both isoforms are processed to yield the same mature subunit.


1.Endoplasmic reticulum stress activates autophagy but not the proteasome in neuronal cells: implications for Alzheimer's disease.Nijholt DA, de Graaf TR, van Haastert ES, Oliveira AO, Berkers CR, Zwart R, Ovaa H, Baas F, Hoozemans JJ, Scheper W.Cell Death Differ. 2011 Jan 21. [Epub ahead of print]

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$ 315.00
Cat# AT3463a
(40 western blots)
Availability: 7-10 days
Bulk Size
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