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>   home   >   Products   >   Primary Antibodies   >   Cell Biology   >   PSMD14 Antibody (monoclonal) (M01)   

PSMD14 Antibody (monoclonal) (M01)

Mouse monoclonal antibody raised against a full length recombinant PSMD14.

     
  • WB - PSMD14 Antibody (monoclonal) (M01) AT3469a
    PSMD14 monoclonal antibody (M01), clone 4A10-E8 Western Blot analysis of PSMD14 expression in A-431 ( Cat # L015V1 ).
  • IF - PSMD14 Antibody (monoclonal) (M01) AT3469a
    Immunofluorescence of monoclonal antibody to PSMD14 on A-431 cell. [antibody concentration 25 ug/ml]
  • E - PSMD14 Antibody (monoclonal) (M01) AT3469a
    Detection limit for recombinant GST tagged PSMD14 is approximately 0.03ng/ml as a capture antibody.
  • SPECIFICATION
  • CITATIONS
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
WB, IF, E
Primary Accession O00487
Other Accession BC009524
Reactivity Human
Host mouse
Clonality Monoclonal
Isotype IgG1 Kappa
Clone Names 4A10-E8
Calculated MW 34577 Da
Additional Information
Gene ID 10213
Other Names 26S proteasome non-ATPase regulatory subunit 14, 3419-, 26S proteasome regulatory subunit RPN11, 26S proteasome-associated PAD1 homolog 1, PSMD14, POH1
Target/Specificity PSMD14 (AAH09524.1, 1 a.a. ~ 95 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
Dilution WB~~1:500~1000
Format Clear, colorless solution in phosphate buffered saline, pH 7.2 .
StorageStore at -20°C or lower. Aliquot to avoid repeated freezing and thawing.
PrecautionsPSMD14 Antibody (monoclonal) (M01) is for research use only and not for use in diagnostic or therapeutic procedures.
Research Areas
Citations (0)

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Background

PSMD14 is a component of the 26S proteasome, a multiprotein complex that degrades proteins targeted for destruction by the ubiquitin pathway (Spataro et al., 1997 [PubMed 9374539]).

References

Knockdown of human deubiquitinase PSMD14 induces cell cycle arrest and senescence. Byrne A, et al. Exp Cell Res, 2010 Jan 15. PMID 19732767.Defining the human deubiquitinating enzyme interaction landscape. Sowa ME, et al. Cell, 2009 Jul 23. PMID 19615732.Relationship between expression of Pad1 homologue and multidrug resistance of idiopathic nephrotic syndrome. Ma Z, et al. Pediatr Int, 2009 Oct. PMID 19419512.Chaperone-mediated pathway of proteasome regulatory particle assembly. Roelofs J, et al. Nature, 2009 Jun 11. PMID 19412159.K63-specific deubiquitination by two JAMM/MPN+ complexes: BRISC-associated Brcc36 and proteasomal Poh1. Cooper EM, et al. EMBO J, 2009 Mar 18. PMID 19214193.

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$ 315.00
Cat# AT3469a
Size:
Quantity:
(40 western blots)
Availability: 7-10 days
Bulk Size
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