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PYCRL Antibody (monoclonal) (M01)

Mouse monoclonal antibody raised against a partial recombinant PYCRL.

     
  • IF - PYCRL Antibody (monoclonal) (M01) AT3505a
    Immunofluorescence of monoclonal antibody to PYCRL on HeLa cell. [antibody concentration 10 ug/ml]
    detail
  • IP - PYCRL Antibody (monoclonal) (M01) AT3505a
    Immunoprecipitation of PYCRL transfected lysate using anti-PYCRL monoclonal antibody and Protein A Magnetic Bead (U0007), and immunoblotted with PYCRL monoclonal antibody.
    detail
  • WB - PYCRL Antibody (monoclonal) (M01) AT3505a
    Antibody Reactive Against Recombinant Protein.Western Blot detection against Immunogen (36.74 KDa) .
    detail
  • WB - PYCRL Antibody (monoclonal) (M01) AT3505a
    PYCRL monoclonal antibody (M01), clone 4F11 Western Blot analysis of PYCRL expression in HeLa ( (Cat # AT3505a )
    detail
  • WB - PYCRL Antibody (monoclonal) (M01) AT3505a
    Western Blot analysis of PYCRL expression in transfected 293T cell line by PYCRL monoclonal antibody (M01), clone 4F11.

    Lane 1: PYCRL transfected lysate (Predicted MW: 28.6 KDa).
    Lane 2: Non-transfected lysate.
    detail
  • E - PYCRL Antibody (monoclonal) (M01) AT3505a
    Detection limit for recombinant GST tagged PYCRL is approximately 0.3ng/ml as a capture antibody.
    detail
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
WB, IF, IP, E
Primary Accession Q53H96
Other Accession NM_023078
Reactivity Human
Host mouse
Clonality Monoclonal
Isotype IgG2a Kappa
Clone Names 4F11
Calculated MW 28663 Da
Additional Information
Gene ID 65263
Other Names Pyrroline-5-carboxylate reductase 3, P5C reductase 3, P5CR 3, Pyrroline-5-carboxylate reductase-like protein, PYCRL
Target/Specificity PYCRL (NP_075566, 32 a.a. ~ 131 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
Dilution WB~~1:500~1000
Format Clear, colorless solution in phosphate buffered saline, pH 7.2 .
StorageStore at -20°C or lower. Aliquot to avoid repeated freezing and thawing.
PrecautionsPYCRL Antibody (monoclonal) (M01) is for research use only and not for use in diagnostic or therapeutic procedures.
Research Areas
Citations (0)
citation

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References

1.Functional specialization in proline biosynthesis of melanoma.De Ingeniis J, Ratnikov B, Richardson AD, Scott DA, Aza-Blanc P, De SK, Kazanov M, Pellecchia M, Ronai Z, Osterman AL, Smith JW.PLoS One. 2012;7(9):e45190. Epub 2012 Sep 14.

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$ 350.00
Cat# AT3505a
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