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RAB7B Antibody (monoclonal) (M01)

Mouse monoclonal antibody raised against a partial recombinant RAB7B.

     
  • IP - RAB7B Antibody (monoclonal) (M01) AT3533a
    Immunoprecipitation of RAB7B transfected lysate using anti-RAB7B monoclonal antibody and Protein A Magnetic Bead (U0007), and immunoblotted with RAB7B MaxPab rabbit polyclonal antibody.
    detail
  • WB - RAB7B Antibody (monoclonal) (M01) AT3533a
    Antibody Reactive Against Recombinant Protein.Western Blot detection against Immunogen (36.74 KDa) .
    detail
  • WB - RAB7B Antibody (monoclonal) (M01) AT3533a
    RAB7B monoclonal antibody (M01), clone 3B3. Western Blot analysis of RAB7B expression in human kidney.
    detail
  • WB - RAB7B Antibody (monoclonal) (M01) AT3533a
    RAB7B monoclonal antibody (M01), clone 3B3. Western Blot analysis of RAB7B expression in A-431((Cat # AT3533a )
    detail
  • WB - RAB7B Antibody (monoclonal) (M01) AT3533a
    Western Blot analysis of RAB7B expression in transfected 293T cell line by RAB7B monoclonal antibody (M01), clone 3B3.

    Lane 1: RAB7B transfected lysate(22.5 KDa).
    Lane 2: Non-transfected lysate.
    detail
  • E - RAB7B Antibody (monoclonal) (M01) AT3533a
    Detection limit for recombinant GST tagged RAB7B is approximately 0.03ng/ml as a capture antibody.
    detail
  • SPECIFICATION
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
WB, IP, E
Primary Accession Q96AH8
Other Accession NM_177403
Reactivity Human
Host mouse
Clonality Monoclonal
Isotype IgG2a Kappa
Clone Names 3B3
Calculated MW 22511 Da
Additional Information
Gene ID 338382
Other Names Ras-related protein Rab-7b, RAB7B
Target/Specificity RAB7B (NP_796377, 100 a.a. ~ 199 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
Dilution WB~~1:500~1000
Format Clear, colorless solution in phosphate buffered saline, pH 7.2 .
StorageStore at -20°C or lower. Aliquot to avoid repeated freezing and thawing.
PrecautionsRAB7B Antibody (monoclonal) (M01) is for research use only and not for use in diagnostic or therapeutic procedures.
Research Areas
Citations (0)
citation

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References

1.Clathrin-dependent mechanisms modulate the subcellular distribution of class C Vps/HOPS tether subunits in polarized and nonpolarized cells.Zlatic SA, Tornieri K, L'hernault SW, Faundez V.Mol Biol Cell. 2011 May;22(10):1699-715. Epub 2011 Mar 16.2.Abnormal localization of leucine-rich repeat kinase 2 to the endosomal-lysosomal compartment in lewy body disease.Higashi S, Moore DJ, Yamamoto R, Minegishi M, Sato K, Togo T, Katsuse O, Uchikado H, Furukawa Y, Hino H, Kosaka K, Emson PC, Wada K, Dawson VL, Dawson TM, Arai H, Iseki E.J Neuropathol Exp Neurol. 2009 Sep;68(9):994-1005.3.GIGYF2 is present in endosomal compartments in the mammalian brains and enhances IGF-1-induced ERK1/2 activation.Higashi S, Iseki E, Minegishi M, Togo T, Kabuta T, Wada K.J Neurochem. 2010 Jul 28. [Epub ahead of print]4.SPE-39 Family Proteins Interact with the HOPS Complex and Function in Lysosomal Delivery.Zhu GD, Salazar G, Zlatic SA, Fiza B, Doucette MM, Heilman CJ, Levey AI, Faundez V, L'hernault SW.Mol Biol Cell. 2009 Feb;20(4):1223-1240. Epub 2008 Dec 24.

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$ 350.00
Cat# AT3533a
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Availability: 7-10 days
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