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>   home   >   Products   >   Primary Antibodies   >   RAD1 Antibody (monoclonal) (M01)   

RAD1 Antibody (monoclonal) (M01)

Mouse monoclonal antibody raised against a partial recombinant RAD1.

  • WB - RAD1 Antibody (monoclonal) (M01) AT3543a
    Antibody Reactive Against Recombinant Protein.Western Blot detection against Immunogen (35.53 KDa) .
  • WB - RAD1 Antibody (monoclonal) (M01) AT3543a
    Western Blot analysis of RAD1 expression in transfected 293T cell line by RAD1 monoclonal antibody (M01), clone 1G2.

    Lane 1: RAD1 transfected lysate(32.43 KDa).
    Lane 2: Non-transfected lysate.
  • IHC - RAD1 Antibody (monoclonal) (M01) AT3543a
    Immunoperoxidase of monoclonal antibody to RAD1 on formalin-fixed paraffin-embedded human lateral ventricle wall. [antibody concentration 3 ug/ml]
  • E - RAD1 Antibody (monoclonal) (M01) AT3543a
    Detection limit for recombinant GST tagged RAD1 is approximately 0.3ng/ml as a capture antibody.
  • WB - RAD1 Antibody (monoclonal) (M01) AT3543a
    Western blot analysis of RAD1 over-expressed 293 cell line, cotransfected with RAD1 Validated Chimera RNAi ( (Cat # AT3543a )
Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary Accession O60671
Other Accession BC006837
Reactivity Human
Host mouse
Clonality Monoclonal
Isotype IgG3 Kappa
Clone Names 1G2
Calculated MW 31827 Da
Additional Information
Gene ID 5810
Other Names Cell cycle checkpoint protein RAD1, hRAD1, DNA repair exonuclease rad1 homolog, Rad1-like DNA damage checkpoint protein, RAD1, REC1
Target/Specificity RAD1 (AAH06837, 1 a.a. ~ 90 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
Dilution WB~~1:500~1000
Format Clear, colorless solution in phosphate buffered saline, pH 7.2 .
StorageStore at -20°C or lower. Aliquot to avoid repeated freezing and thawing.
PrecautionsRAD1 Antibody (monoclonal) (M01) is for research use only and not for use in diagnostic or therapeutic procedures.
Citations (0)

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This gene encodes a component of a heterotrimeric cell cycle checkpoint complex, known as the 9-1-1 complex, that is activated to stop cell cycle progression in response to DNA damage or incomplete DNA replication. The 9-1-1 complex is recruited by RAD17 to affected sites where it may attract specialized DNA polymerases and other DNA repair effectors. Alternatively spliced transcript variants of this gene have been described.


An approach based on a genome-wide association study reveals candidate loci for narcolepsy. Shimada M, et al. Hum Genet, 2010 Oct. PMID 20677014.Interaction between human mismatch repair recognition proteins and checkpoint sensor Rad9-Rad1-Hus1. Bai H, et al. DNA Repair (Amst), 2010 May 4. PMID 20188637.Repair activities of human 8-oxoguanine DNA glycosylase are stimulated by the interaction with human checkpoint sensor Rad9-Rad1-Hus1 complex. Park MJ, et al. DNA Repair (Amst), 2009 Oct 2. PMID 19615952.Structure and functional implications of the human rad9-hus1-rad1 cell cycle checkpoint complex. Xu M, et al. J Biol Chem, 2009 Jul 31. PMID 19535328.The DNA binding domain of human DNA ligase I interacts with both nicked DNA and the DNA sliding clamps, PCNA and hRad9-hRad1-hHus1. Song W, et al. DNA Repair (Amst), 2009 Aug 6. PMID 19523882.

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$ 315.00
Cat# AT3543a
(40 western blots)
Availability: 7-10 days
Bulk Size
Seasonal Special on Bulk Order
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