RAD18 Antibody (monoclonal) (M01)
Mouse monoclonal antibody raised against a partial recombinant RAD18.
|Application ||WB, IHC, IF|
|Calculated MW||56223 Da|
|Other Names||E3 ubiquitin-protein ligase RAD18, 632-, Postreplication repair protein RAD18, hHR18, hRAD18, RING finger protein 73, RAD18, RNF73|
|Target/Specificity||RAD18 (NP_064550, 332 a.a. ~ 430 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.|
|Format||Clear, colorless solution in phosphate buffered saline, pH 7.2 .|
|Storage||Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.|
|Precautions||RAD18 Antibody (monoclonal) (M01) is for research use only and not for use in diagnostic or therapeutic procedures.|
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Provided below are standard protocols that you may find useful for product applications.
The protein encoded by this gene is highly similar to S. cerevisiae DNA damage repair protein Rad18. Yeast Rad18 functions through its interaction with Rad6, which is an ubiquitin-conjugating enzyme required for post-replication repair of damaged DNA. Similar to its yeast counterpart, this protein is able to interact with the human homolog of yeast Rad6 protein through a conserved ring-finger motif. Mutation of this motif results in defective replication of UV-damaged DNA and hypersensitivity to multiple mutagens.
1.The ubiquitin specific protease USP34 promotes ubiquitin signaling at DNA double-strand breaks.Sy SM, Jiang J, O WS, Deng Y, Huen MSNucleic Acids Res. 2013 Jul 17.2.Phosphorylation of human INO80 is involved in DNA damage toleranceKato D, Waki M, Umezawa M, Aoki Y, Utsugi T, Ohtsu M, Murakami Y.Biochem Biophys Res Commun. 2011 Dec 7. [Epub ahead of print]3.Elevated expression of Rad18 regulates melanoma cell proliferation.Wong RP, Aguissa-Toure AH, Wani AA, Khosravi S, Martinka M, Martinka M, Li G.Pigment Cell Melanoma Res. 2011 Dec 6. doi: 10.1111/j.1755-148X.2011.00948.x. [Epub ahead of print]4.NBS1 Recruits RAD18 via a RAD6-like Domain and Regulates Pol ?b-Dependent Translesion DNA Synthesis.Yanagihara H, Kobayashi J, Tateishi S, Kato A, Matsuura S, Tauchi H, Yamada K, Takezawa J, Sugasawa K, Masutani C, Hanaoka F, Weemaes CM, Mori T, Zou L, Komatsu K.Mol Cell. 2011 Sep 2;43(5):788-97.5.Tumour suppressor ING1b maintains genomic stability upon replication stress.Wong RP, Lin H, Khosravi S, Piche B, Jafarnejad SM, Chen DW, Li G.Nucleic Acids Res. 2011 Jan 11. [Epub ahead of print]6.WRN participates in translesion synthesis pathway through interaction with NBS1.Kobayashi J, Okui M, Asaithamby A, Burma S, Chen BP, Tanimoto K, Matsuura S, Komatsu K, Chen DJ.Mech Ageing Dev. 2010 Jun 17. [Epub ahead of print]7.Differential roles for DNA Polymerases Eta, Zeta and REV1 in lesion bypass of intrastrand versus interstrand DNA cross-links.Hicks JK, Chute CL, Paulsen MT, Ragland RL, Howlett NG, Gueranger Q, Glover TW, Canman CE.Mol Cell Biol. 2009 Dec 22. [Epub ahead of print]
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