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>   home   >   Products   >   Primary Antibodies   >   Cell Biology   >   RAD18 Antibody (monoclonal) (M01)   

RAD18 Antibody (monoclonal) (M01)

Mouse monoclonal antibody raised against a partial recombinant RAD18.

  • WB - RAD18 Antibody (monoclonal) (M01) AT3545a
    Antibody Reactive Against Recombinant Protein.Western Blot detection against Immunogen (36.63 KDa) .
  • WB - RAD18 Antibody (monoclonal) (M01) AT3545a
    RAD18 monoclonal antibody (M01), clone 3H7. Western Blot analysis of RAD18 expression in PC-12 ( (Cat # AT3545a )
  • WB - RAD18 Antibody (monoclonal) (M01) AT3545a
    RAD18 monoclonal antibody (M01), clone 3H7 Western Blot analysis of RAD18 expression in Hela S3 NE ( (Cat # AT3545a )
  • WB - RAD18 Antibody (monoclonal) (M01) AT3545a
    Western Blot analysis of RAD18 expression in transfected 293T cell line by RAD18 monoclonal antibody (M01), clone 3H7.

    Lane 1: RAD18 transfected lysate(56.2 KDa).
    Lane 2: Non-transfected lysate.
  • IHC - RAD18 Antibody (monoclonal) (M01) AT3545a
    Immunoperoxidase of monoclonal antibody to RAD18 on formalin-fixed paraffin-embedded human testis. [antibody concentration 1.5 ug/ml]
  • IF - RAD18 Antibody (monoclonal) (M01) AT3545a
    Immunofluorescence of monoclonal antibody to RAD18 on HeLa cell. [antibody concentration 25 ug/ml]
  • E - RAD18 Antibody (monoclonal) (M01) AT3545a
    Detection limit for recombinant GST tagged RAD18 is approximately 0.1ng/ml as a capture antibody.
Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary Accession Q9NS91
Other Accession NM_020165
Reactivity Human, Rat
Host Mouse
Clonality Monoclonal
Isotype IgG2b Kappa
Clone Names 3H7
Calculated MW 56223 Da
Additional Information
Gene ID 56852
Other Names E3 ubiquitin-protein ligase RAD18, 632-, Postreplication repair protein RAD18, hHR18, hRAD18, RING finger protein 73, RAD18, RNF73
Target/Specificity RAD18 (NP_064550, 332 a.a. ~ 430 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
Dilution WB~~1:500~1000
Format Clear, colorless solution in phosphate buffered saline, pH 7.2 .
StorageStore at -20°C or lower. Aliquot to avoid repeated freezing and thawing.
PrecautionsRAD18 Antibody (monoclonal) (M01) is for research use only and not for use in diagnostic or therapeutic procedures.
Research Areas
Citations (0)

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The protein encoded by this gene is highly similar to S. cerevisiae DNA damage repair protein Rad18. Yeast Rad18 functions through its interaction with Rad6, which is an ubiquitin-conjugating enzyme required for post-replication repair of damaged DNA. Similar to its yeast counterpart, this protein is able to interact with the human homolog of yeast Rad6 protein through a conserved ring-finger motif. Mutation of this motif results in defective replication of UV-damaged DNA and hypersensitivity to multiple mutagens.


1.The ubiquitin specific protease USP34 promotes ubiquitin signaling at DNA double-strand breaks.Sy SM, Jiang J, O WS, Deng Y, Huen MSNucleic Acids Res. 2013 Jul 17.2.Phosphorylation of human INO80 is involved in DNA damage toleranceKato D, Waki M, Umezawa M, Aoki Y, Utsugi T, Ohtsu M, Murakami Y.Biochem Biophys Res Commun. 2011 Dec 7. [Epub ahead of print]3.Elevated expression of Rad18 regulates melanoma cell proliferation.Wong RP, Aguissa-Toure AH, Wani AA, Khosravi S, Martinka M, Martinka M, Li G.Pigment Cell Melanoma Res. 2011 Dec 6. doi: 10.1111/j.1755-148X.2011.00948.x. [Epub ahead of print]4.NBS1 Recruits RAD18 via a RAD6-like Domain and Regulates Pol ?b-Dependent Translesion DNA Synthesis.Yanagihara H, Kobayashi J, Tateishi S, Kato A, Matsuura S, Tauchi H, Yamada K, Takezawa J, Sugasawa K, Masutani C, Hanaoka F, Weemaes CM, Mori T, Zou L, Komatsu K.Mol Cell. 2011 Sep 2;43(5):788-97.5.Tumour suppressor ING1b maintains genomic stability upon replication stress.Wong RP, Lin H, Khosravi S, Piche B, Jafarnejad SM, Chen DW, Li G.Nucleic Acids Res. 2011 Jan 11. [Epub ahead of print]6.WRN participates in translesion synthesis pathway through interaction with NBS1.Kobayashi J, Okui M, Asaithamby A, Burma S, Chen BP, Tanimoto K, Matsuura S, Komatsu K, Chen DJ.Mech Ageing Dev. 2010 Jun 17. [Epub ahead of print]7.Differential roles for DNA Polymerases Eta, Zeta and REV1 in lesion bypass of intrastrand versus interstrand DNA cross-links.Hicks JK, Chute CL, Paulsen MT, Ragland RL, Howlett NG, Gueranger Q, Glover TW, Canman CE.Mol Cell Biol. 2009 Dec 22. [Epub ahead of print]

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$ 315.00
Cat# AT3545a
(40 western blots)
Availability: 7-10 days
Bulk Size
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