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RANBP5 Antibody (monoclonal) (M01)

Mouse monoclonal antibody raised against a partial recombinant RANBP5.

     
  • WB - RANBP5 Antibody (monoclonal) (M01) AT3561a
    Antibody Reactive Against Recombinant Protein.Western Blot detection against Immunogen (36.74 KDa) .
    detail
  • WB - RANBP5 Antibody (monoclonal) (M01) AT3561a
    RANBP5 monoclonal antibody (M01), clone 1C4 Western Blot analysis of RANBP5 expression in HeLa ( (Cat # AT3561a )
    detail
  • WB - RANBP5 Antibody (monoclonal) (M01) AT3561a
    RANBP5 monoclonal antibody (M01), clone 1C4. Western Blot analysis of IPO5 expression in PC-12.
    detail
  • WB - RANBP5 Antibody (monoclonal) (M01) AT3561a
    RANBP5 monoclonal antibody (M01), clone 1C4. Western Blot analysis of IPO5 expression in Raw 264.7.
    detail
  • WB - RANBP5 Antibody (monoclonal) (M01) AT3561a
    Western Blot analysis of IPO5 expression in transfected 293T cell line by RANBP5 monoclonal antibody (M01), clone 1C4.

    Lane 1: IPO5 transfected lysate (Predicted MW: 125.6 KDa).
    Lane 2: Non-transfected lysate.
    detail
  • IHC - RANBP5 Antibody (monoclonal) (M01) AT3561a
    Immunoperoxidase of monoclonal antibody to RANBP5 on formalin-fixed paraffin-embedded human pancreas. [antibody concentration 3 ug/ml]
    detail
  • E - RANBP5 Antibody (monoclonal) (M01) AT3561a
    Detection limit for recombinant GST tagged RANBP5 is approximately 0.03ng/ml as a capture antibody.
    detail
  • SPECIFICATION
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  • BACKGROUND
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
WB, IHC
Primary Accession O00410
Other Accession BC001497
Reactivity Human, Mouse, Rat
Host mouse
Clonality Monoclonal
Isotype IgG1 Kappa
Clone Names 1C4
Calculated MW 123630 Da
Additional Information
Gene ID 3843
Other Names Importin-5, Imp5, Importin subunit beta-3, Karyopherin beta-3, Ran-binding protein 5, RanBP5, IPO5, KPNB3, RANBP5
Target/Specificity RANBP5 (AAH01497, 1 a.a. ~ 100 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
Dilution WB~~1:500~1000
Format Clear, colorless solution in phosphate buffered saline, pH 7.2 .
StorageStore at -20°C or lower. Aliquot to avoid repeated freezing and thawing.
PrecautionsRANBP5 Antibody (monoclonal) (M01) is for research use only and not for use in diagnostic or therapeutic procedures.
Research Areas
Citations (0)
citation

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Background

Nucleocytoplasmic transport, a signal- and energy-dependent process, takes place through nuclear pore complexes embedded in the nuclear envelope. The import of proteins containing a nuclear localization signal (NLS) requires the NLS import receptor, a heterodimer of importin alpha and beta subunits also known as karyopherins. Importin alpha binds the NLS-containing cargo in the cytoplasm and importin beta docks the complex at the cytoplasmic side of the nuclear pore complex. In the presence of nucleoside triphosphates and the small GTP binding protein Ran, the complex moves into the nuclear pore complex and the importin subunits dissociate. Importin alpha enters the nucleoplasm with its passenger protein and importin beta remains at the pore. Interactions between importin beta and the FG repeats of nucleoporins are essential in translocation through the pore complex. The protein encoded by this gene is a member of the importin beta family.

References

1.Localization of retinitis pigmentosa 2 to cilia is regulated by Importin {beta}2.Hurd TW, Fan S, Margolis BL.J Cell Sci. 2011 Mar 1;124(Pt 5):718-26. Epub 2011 Feb 1.

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$ 350.00
Cat# AT3561a
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