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RAP1A Antibody (monoclonal) (M01)

Mouse monoclonal antibody raised against a full length recombinant RAP1A.

     
  • WB - RAP1A Antibody (monoclonal) (M01) AT3562a
    Antibody Reactive Against Recombinant Protein.Western Blot detection against Immunogen (45.98 KDa) .
    detail
  • E - RAP1A Antibody (monoclonal) (M01) AT3562a
    Detection limit for recombinant GST tagged RAP1A is approximately 1ng/ml as a capture antibody.
    detail
  •  - RAP1A Antibody (monoclonal) (M01) AT3562a
    Proximity Ligation Analysis of protein-protein interactions between RASGRP4 and RAP1A HeLa cells were stained with anti-RASGRP4 rabbit purified polyclonal 1:1200 and anti-RAP1A mouse monoclonal antibody 1:50. Each red dot represents the detection of protein-protein interaction complex, and nuclei were counterstained with DAPI (blue).
    detail
  • SPECIFICATION
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
WB, E
Primary Accession P62834
Other Accession BC014086
Reactivity Human
Host mouse
Clonality Monoclonal
Isotype IgG2a Kappa
Clone Names 30
Calculated MW 20987 Da
Additional Information
Gene ID 5906
Other Names Ras-related protein Rap-1A, C21KG, G-22K, GTP-binding protein smg p21A, Ras-related protein Krev-1, RAP1A, KREV1
Target/Specificity RAP1A (AAH14086.1, 1 a.a. ~ 184 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
Dilution WB~~1:500~1000
Format Clear, colorless solution in phosphate buffered saline, pH 7.2 .
StorageStore at -20°C or lower. Aliquot to avoid repeated freezing and thawing.
PrecautionsRAP1A Antibody (monoclonal) (M01) is for research use only and not for use in diagnostic or therapeutic procedures.
Research Areas
Citations (0)
citation

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Background

The product of this gene belongs to the family of RAS-related proteins. These proteins share approximately 50% amino acid identity with the classical RAS proteins and have numerous structural features in common. The most striking difference between RAP proteins and RAS proteins resides in their 61st amino acid: glutamine in RAS is replaced by threonine in RAP proteins. The product of this gene counteracts the mitogenic function of RAS because it can interact with RAS GAPs and RAF in a competitive manner. Two transcript variants encoding the same protein have been identified for this gene.

References

1.Overexpression of Rap-1A Indicates a Poor Prognosis for Oral Cavity Squamous Cell Carcinoma and Promotes Tumor Cell Invasion via Aurora-A Modulation.Chen CH, Chuang HC, Huang CC, Fang FM, Huang HY, Tasi HT, Su LJ, Shiu LY, Leu S, Chien CY.Am J Pathol. 2012 Dec 3. pii: S0002-9440(12)00817-6. doi: 10.1016/j.ajpath.2012.10.023. [Epub ahead of print]

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$ 350.00
Cat# AT3562a
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Availability: 7-10 days
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