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RBAF600 Antibody (monoclonal) (M01)

Mouse monoclonal antibody raised against a partial recombinant RBAF600.

     
  • IF - RBAF600 Antibody (monoclonal) (M01) AT3584a
    Immunofluorescence of monoclonal antibody to RBAF600 on HeLa cell. [antibody concentration 10 ug/ml]
    detail
  • WB - RBAF600 Antibody (monoclonal) (M01) AT3584a
    Antibody Reactive Against Recombinant Protein.Western Blot detection against Immunogen (36.41 KDa) .
    detail
  • E - RBAF600 Antibody (monoclonal) (M01) AT3584a
    Detection limit for recombinant GST tagged RBAF600 is approximately 0.1ng/ml as a capture antibody.
    detail
  • SPECIFICATION
  • CITATIONS
  • PROTOCOLS
  • BACKGROUND
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
WB, IF, E
Primary Accession Q5T4S7
Other Accession NM_020765
Reactivity Human
Host Mouse
Clonality Monoclonal
Isotype IgG2b Kappa
Clone Names 2D12
Calculated MW 573841 Da
Additional Information
Gene ID 23352
Other Names E3 ubiquitin-protein ligase UBR4, 632-, 600 kDa retinoblastoma protein-associated factor, N-recognin-4, Retinoblastoma-associated factor of 600 kDa, RBAF600, p600, Zinc finger UBR1-type protein 1, UBR4, KIAA0462, KIAA1307, RBAF600, ZUBR1
Target/Specificity RBAF600 (NP_065816, 94 a.a. ~ 190 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
Dilution WB~~1:500~1000
Format Clear, colorless solution in phosphate buffered saline, pH 7.2 .
StorageStore at -20°C or lower. Aliquot to avoid repeated freezing and thawing.
PrecautionsRBAF600 Antibody (monoclonal) (M01) is for research use only and not for use in diagnostic or therapeutic procedures.
Research Areas
Citations (0)
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Background

The protein encoded by this gene is an E3 ubiquitin-protein ligase that interacts with the retinoblastoma-associated protein in the nucleus and with calcium-bound calmodulin in the cytoplasm. The encoded protein appears to be a cytoskeletal component in the cytoplasm and part of the chromatin scaffold in the nucleus. In addition, this protein is a target of the human papillomavirus type 16 E7 oncoprotein.

References

Personalized smoking cessation: interactions between nicotine dose, dependence and quit-success genotype score. Rose JE, et al. Mol Med, 2010 Jul-Aug. PMID 20379614.Large-scale mapping of human protein-protein interactions by mass spectrometry. Ewing RM, et al. Mol Syst Biol, 2007. PMID 17353931.The response of autologous T cells to a human melanoma is dominated by mutated neoantigens. Lennerz V, et al. Proc Natl Acad Sci U S A, 2005 Nov 1. PMID 16247014.p600, a unique protein required for membrane morphogenesis and cell survival. Nakatani Y, et al. Proc Natl Acad Sci U S A, 2005 Oct 18. PMID 16214886.Association of the human papillomavirus type 16 E7 oncoprotein with the 600-kDa retinoblastoma protein-associated factor, p600. Huh KW, et al. Proc Natl Acad Sci U S A, 2005 Aug 9. PMID 16061792.

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$ 350.00
Cat# AT3584a
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