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>   home   >   Products   >   Primary Antibodies   >   RFC4 Antibody (monoclonal) (M01)   

RFC4 Antibody (monoclonal) (M01)

Mouse monoclonal antibody raised against a partial recombinant RFC4.

  • WB - RFC4 Antibody (monoclonal) (M01) AT3621a
    Antibody Reactive Against Recombinant Protein.Western Blot detection against Immunogen (37.73 KDa) .
  • WB - RFC4 Antibody (monoclonal) (M01) AT3621a
    RFC4 monoclonal antibody (M01), clone 1C12 Western Blot analysis of RFC4 expression in K-562 ( (Cat # AT3621a )
  • WB - RFC4 Antibody (monoclonal) (M01) AT3621a
    Western Blot analysis of RFC4 expression in transfected 293T cell line by RFC4 monoclonal antibody (M01), clone 1C12.

    Lane 1: RFC4 transfected lysate(39.7 KDa).
    Lane 2: Non-transfected lysate.
  • IHC - RFC4 Antibody (monoclonal) (M01) AT3621a
    Immunoperoxidase of monoclonal antibody to RFC4 on formalin-fixed paraffin-embedded human tonsil. [antibody concentration 3 ug/ml]
  • E - RFC4 Antibody (monoclonal) (M01) AT3621a
    Detection limit for recombinant GST tagged RFC4 is approximately 0.3ng/ml as a capture antibody.
  • WB - RFC4 Antibody (monoclonal) (M01) AT3621a
    Western blot analysis of RFC4 over-expressed 293 cell line, cotransfected with RFC4 Validated Chimera RNAi ( (Cat # AT3621a )
Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary Accession P35249
Other Accession BC017452
Reactivity Human
Host mouse
Clonality Monoclonal
Isotype IgG2a Kappa
Clone Names 1C12
Calculated MW 39682 Da
Additional Information
Gene ID 5984
Other Names Replication factor C subunit 4, Activator 1 37 kDa subunit, A1 37 kDa subunit, Activator 1 subunit 4, Replication factor C 37 kDa subunit, RF-C 37 kDa subunit, RFC37, RFC4
Target/Specificity RFC4 (AAH17452, 254 a.a. ~ 363 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
Dilution WB~~1:500~1000
Format Clear, colorless solution in phosphate buffered saline, pH 7.2 .
StorageStore at -20°C or lower. Aliquot to avoid repeated freezing and thawing.
PrecautionsRFC4 Antibody (monoclonal) (M01) is for research use only and not for use in diagnostic or therapeutic procedures.
Citations (0)

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The elongation of primed DNA templates by DNA polymerase delta and DNA polymerase epsilon requires the accessory proteins proliferating cell nuclear antigen (PCNA) and replication factor C (RFC). RFC, also named activator 1, is a protein complex consisting of five distinct subunits of 140, 40, 38, 37, and 36 kD. This gene encodes the 37 kD subunit. This subunit forms a core complex with the 36 and 40 kDa subunits. The core complex possesses DNA-dependent ATPase activity, which was found to be stimulated by PCNA in an in vitro system. Alternatively spliced transcript variants encoding the same protein have been reported.


Polymorphisms in the base excision repair pathway and graft-versus-host disease. Arora M, et al. Leukemia, 2010 Aug. PMID 20574454.Variation within DNA repair pathway genes and risk of multiple sclerosis. Briggs FB, et al. Am J Epidemiol, 2010 Jul 15. PMID 20522537.Association between genetic variants in the base excision repair pathway and outcomes after hematopoietic cell transplantations. Thyagarajan B, et al. Biol Blood Marrow Transplant, 2010 Aug. PMID 20226869.A genome-wide association study reveals variants in ARL15 that influence adiponectin levels. Richards JB, et al. PLoS Genet, 2009 Dec. PMID 20011104.Defining the human deubiquitinating enzyme interaction landscape. Sowa ME, et al. Cell, 2009 Jul 23. PMID 19615732.

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$ 315.00
Cat# AT3621a
(40 western blots)
Availability: 7-10 days
Bulk Size
Seasonal Special on Bulk Order
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