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RFFL Antibody (monoclonal) (M01)

Mouse monoclonal antibody raised against a partial recombinant RFFL.

  • WB - RFFL Antibody (monoclonal) (M01) AT3622a
    Antibody Reactive Against Recombinant Protein.Western Blot detection against Immunogen (36.52 KDa) .
  • WB - RFFL Antibody (monoclonal) (M01) AT3622a
    Western Blot analysis of RFFL expression in transfected 293T cell line by RFFL monoclonal antibody (M01), clone 3A4.

    Lane 1: RFFL transfected lysate(36.6 KDa).
    Lane 2: Non-transfected lysate.
  • E - RFFL Antibody (monoclonal) (M01) AT3622a
    Detection limit for recombinant GST tagged RFFL is 0.03 ng/ml as a capture antibody.
  • WB - RFFL Antibody (monoclonal) (M01) AT3622a
    Western blot analysis of RFFL over-expressed 293 cell line, cotransfected with RFFL Validated Chimera RNAi ( (Cat # AT3622a )
Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary Accession Q8WZ73
Other Accession NM_001017368
Reactivity Human
Host mouse
Clonality Monoclonal
Isotype IgG2a Kappa
Clone Names 3A4
Calculated MW 40514 Da
Additional Information
Gene ID 117584
Other Names E3 ubiquitin-protein ligase rififylin, 632-, Caspase regulator CARP2, Caspases-8 and -10-associated RING finger protein 2, CARP-2, FYVE-RING finger protein Sakura, Fring, RING finger and FYVE-like domain-containing protein 1, RING finger protein 189, RING finger protein 34-like, RFFL (HGNC:24821), RNF189, RNF34L
Target/Specificity RFFL (NP_001017368, 2 a.a. ~ 99 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
Dilution WB~~1:500~1000
Format Clear, colorless solution in phosphate buffered saline, pH 7.2 .
StorageStore at -20°C or lower. Aliquot to avoid repeated freezing and thawing.
PrecautionsRFFL Antibody (monoclonal) (M01) is for research use only and not for use in diagnostic or therapeutic procedures.
Research Areas
Citations (0)

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An approach based on a genome-wide association study reveals candidate loci for narcolepsy. Shimada M, et al. Hum Genet, 2010 Oct. PMID 20677014.Association between genetic variants in VEGF, ERCC3 and occupational benzene haematotoxicity. Hosgood HD 3rd, et al. Occup Environ Med, 2009 Dec. PMID 19773279.Common variants at ten loci influence QT interval duration in the QTGEN Study. Newton-Cheh C, et al. Nat Genet, 2009 Apr. PMID 19305408.CARP-2 is an endosome-associated ubiquitin ligase for RIP and regulates TNF-induced NF-kappaB activation. Liao W, et al. Curr Biol, 2008 May 6. PMID 18450452.CARPs enhance p53 turnover by degrading 14-3-3sigma and stabilizing MDM2. Yang W, et al. Cell Cycle, 2008 Mar 1. PMID 18382127.

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$ 315.00
Cat# AT3622a
(40 western blots)
Availability: 7-10 days
Bulk Size
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