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RGS20 Antibody (monoclonal) (M04)

Mouse monoclonal antibody raised against a partial recombinant RGS20.

     
  • IF - RGS20 Antibody (monoclonal) (M04) AT3629a
    Immunofluorescence of monoclonal antibody to RGS20 on HeLa cell . [antibody concentration 10 ug/ml]
    detail
  • WB - RGS20 Antibody (monoclonal) (M04) AT3629a
    Antibody Reactive Against Recombinant Protein.Western Blot detection against Immunogen (36.63 KDa) .
    detail
  • WB - RGS20 Antibody (monoclonal) (M04) AT3629a
    RGS20 monoclonal antibody (M04), clone 3E10. Western Blot analysis of RGS20 expression in NIH/3T3 ( (Cat # AT3629a )
    detail
  • E - RGS20 Antibody (monoclonal) (M04) AT3629a
    Detection limit for recombinant GST tagged RGS20 is 0.3 ng/ml as a capture antibody.
    detail
  • SPECIFICATION
  • CITATIONS
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
WB, IF, E
Primary Accession O76081
Other Accession NM_003702
Reactivity Human, Mouse
Host Mouse
Clonality Monoclonal
Isotype IgG2a Kappa
Clone Names 3E11
Calculated MW 43692 Da
Additional Information
Gene ID 8601
Other Names Regulator of G-protein signaling 20, RGS20, Gz-selective GTPase-activating protein, G(z)GAP, Gz-GAP, Regulator of G-protein signaling Z1, Regulator of Gz-selective protein signaling 1, RGS20, RGSZ1, ZGAP1
Target/Specificity RGS20 (NP_003693, 78 a.a. ~ 176 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
Dilution WB~~1:500~1000
Format Clear, colorless solution in phosphate buffered saline, pH 7.2 .
StorageStore at -20°C or lower. Aliquot to avoid repeated freezing and thawing.
PrecautionsRGS20 Antibody (monoclonal) (M04) is for research use only and not for use in diagnostic or therapeutic procedures.
Research Areas
Citations (0)
citation

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Background

Regulator of G protein signaling (RGS) proteins are regulatory and structural components of G protein-coupled receptor complexes. RGS proteins are GTPase-activating proteins for Gi (see GNAI1; MIM 139310) and Gq (see GNAQ; MIM 600998) class G-alpha proteins. They accelerate transit through the cycle of GTP binding and hydrolysis and thereby accelerate signaling kinetics and termination.

References

Genetic Variations in Regulator of G-Protein Signaling Genes as Susceptibility Loci for Second Primary Tumor/Recurrence in Head and Neck Squamous Cell Carcinoma. Wang J, et al. Carcinogenesis, 2010 Jul 12. PMID 20627871.Galphao/i-stimulated proteosomal degradation of RGS20: a mechanism for temporal integration of Gs and Gi pathways. Pagano M, et al. Cell Signal, 2008 Jun. PMID 18407463.Identification of hypertension-susceptibility genes and pathways by a systemic multiple candidate gene approach: the millennium genome project for hypertension. Kohara K, et al. Hypertens Res, 2008 Feb. PMID 18360038.Systematic identification of SH3 domain-mediated human protein-protein interactions by peptide array target screening. Wu C, et al. Proteomics, 2007 Jun. PMID 17474147.Large-scale mapping of human protein-protein interactions by mass spectrometry. Ewing RM, et al. Mol Syst Biol, 2007. PMID 17353931.

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$ 350.00
Cat# AT3629a
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