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>   home   >   Products   >   Primary Antibodies   >   RNF12 Antibody (monoclonal) (M01)   

RNF12 Antibody (monoclonal) (M01)

Mouse monoclonal antibody raised against a partial recombinant RNF12.

  • WB - RNF12 Antibody (monoclonal) (M01) AT3658a
    Antibody Reactive Against Recombinant Protein.Western Blot detection against Immunogen (34.87 KDa) .
  • WB - RNF12 Antibody (monoclonal) (M01) AT3658a
    RNF12 monoclonal antibody (M01), clone 1G10. Western Blot analysis of RNF12 expression in Hela S3 NE.
  • WB - RNF12 Antibody (monoclonal) (M01) AT3658a
    Western Blot analysis of RNF12 expression in transfected 293T cell line by RNF12 monoclonal antibody (M01), clone 1G10.

    Lane 1: RNF12 transfected lysate (Predicted MW: 68.5 KDa).
    Lane 2: Non-transfected lysate.
  • IF - RNF12 Antibody (monoclonal) (M01) AT3658a
    Immunofluorescence of monoclonal antibody to RNF12 on HeLa cell. [antibody concentration 30 ug/ml]
  • IP - RNF12 Antibody (monoclonal) (M01) AT3658a
    Immunoprecipitation of RNF12 transfected lysate using anti-RNF12 monoclonal antibody and Protein A Magnetic Bead (U0007), and immunoblotted with RNF12 MaxPab rabbit polyclonal antibody.
  • E - RNF12 Antibody (monoclonal) (M01) AT3658a
    Detection limit for recombinant GST tagged RNF12 is approximately 0.03ng/ml as a capture antibody.
Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary Accession Q9NVW2
Other Accession NM_016120
Reactivity Human
Host Mouse
Clonality Monoclonal
Isotype IgG2a Kappa
Clone Names 1G10
Calculated MW 68549 Da
Additional Information
Gene ID 51132
Other Names E3 ubiquitin-protein ligase RLIM, 632-, LIM domain-interacting RING finger protein, RING finger LIM domain-binding protein, R-LIM, RING finger protein 12, Renal carcinoma antigen NY-REN-43, RLIM, RNF12
Target/Specificity RNF12 (NP_057204, 1 a.a. ~ 83 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
Dilution WB~~1:500~1000
Format Clear, colorless solution in phosphate buffered saline, pH 7.2 .
StorageStore at -20°C or lower. Aliquot to avoid repeated freezing and thawing.
PrecautionsRNF12 Antibody (monoclonal) (M01) is for research use only and not for use in diagnostic or therapeutic procedures.
Citations (0)

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The protein encoded by this gene is a RING-H2 zinc finger protein. It has been shown to be an E3 ubiquitin protein ligase that targets LIM domain binding 1 (LDB1/CLIM), and causes proteasome-dependent degradation of LDB1. This protein and LDB1 are co-repressors of LHX1/LIM-1, a homeodomain transcription factor. Multiple alternatively spliced variants, encoding the same protein, have been identified.


1.Nuclear receptor NR4A1 promotes breast cancer invasion and metastasis by activating TGF-β signalling.Zhou F, Drabsch Y, Dekker TJ, de Vinuesa AG, Li Y, Hawinkels LJ, Sheppard KA, Goumans MJ, Luwor RB, de Vries CJ, Mesker WE, Tollenaar RA, Devilee P, Lu CX, Zhu H, Zhang L, Dijke PTNat Commun. 2014 Mar 3;5:3388. doi: 10.1038/ncomms4388.

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$ 315.00
Cat# AT3658a
(40 western blots)
Availability: 7-10 days
Bulk Size
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