RNF14 Antibody (monoclonal) (M01)
Mouse monoclonal antibody raised against a partial recombinant RNF14.
|Application ||WB, IF, E|
|Calculated MW||53837 Da|
|Other Names||E3 ubiquitin-protein ligase RNF14, 632-, Androgen receptor-associated protein 54, HFB30, RING finger protein 14, Triad2 protein, RNF14, ARA54|
|Target/Specificity||RNF14 (NP_004281, 217 a.a. ~ 316 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.|
|Format||Clear, colorless solution in phosphate buffered saline, pH 7.2 .|
|Storage||Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.|
|Precautions||RNF14 Antibody (monoclonal) (M01) is for research use only and not for use in diagnostic or therapeutic procedures.|
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The protein encoded by this gene contains a RING zinc finger, a motif known to be involved in protein-protein interactions. This protein interacts with androgen receptor (AR) and may function as a coactivator that induces AR target gene expression in prostate. A dominant negative mutant of this gene has been demonstrated to inhibit the AR-mediated growth of prostate cancer. This protein also interacts with class III ubiquitin-conjugating enzymes (E2s) and may act as a ubiquitin-ligase (E3) in the ubiquitination of certain nuclear proteins. Five alternatively spliced transcript variants encoding two distinct isoforms have been reported.
Regulation of androgen receptor transcriptional activity and specificity by RNF6-induced ubiquitination. Xu K, et al. Cancer Cell, 2009 Apr 7. PMID 19345326.Expression of androgen receptor co-regulators in the testes of men with azoospermia. Lan KC, et al. Fertil Steril, 2008 May. PMID 17919607.ARA54 is involved in transcriptional regulation of the cyclin D1 gene in human cancer cells. Kikuchi H, et al. Carcinogenesis, 2007 Aug. PMID 17510080.Suppression of androgen receptor transactivation and prostate cancer cell growth by heterogeneous nuclear ribonucleoprotein A1 via interaction with androgen receptor coregulator ARA54. Yang Z, et al. Endocrinology, 2007 Mar. PMID 17110431.Transgelin functions as a suppressor via inhibition of ARA54-enhanced androgen receptor transactivation and prostate cancer cell growth. Yang Z, et al. Mol Endocrinol, 2007 Feb. PMID 17082327.
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