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RNF40 Antibody (monoclonal) (M09)

Mouse monoclonal antibody raised against a partial recombinant RNF40.

  • WB - RNF40 Antibody (monoclonal) (M09) AT3676a
    Antibody Reactive Against Recombinant Protein.Western Blot detection against Immunogen (36.63 KDa) .
  • WB - RNF40 Antibody (monoclonal) (M09) AT3676a
    Western Blot analysis of RNF40 expression in transfected 293T cell line by RNF40 monoclonal antibody (M09), clone 1C1.

    Lane 1: RNF40 transfected lysate(113.7 KDa).
    Lane 2: Non-transfected lysate.
  • IF - RNF40 Antibody (monoclonal) (M09) AT3676a
    Immunofluorescence of monoclonal antibody to RNF40 on HeLa cell . [antibody concentration 10 ug/ml]
  • E - RNF40 Antibody (monoclonal) (M09) AT3676a
    Detection limit for recombinant GST tagged RNF40 is approximately 1ng/ml as a capture antibody.
Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary Accession O75150
Other Accession NM_014771
Reactivity Human
Host mouse
Clonality Monoclonal
Isotype IgG2a Kappa
Clone Names 1C1
Calculated MW 113650 Da
Additional Information
Gene ID 9810
Other Names E3 ubiquitin-protein ligase BRE1B, BRE1-B, 632-, 95 kDa retinoblastoma-associated protein, RBP95, RING finger protein 40, RNF40, BRE1B, KIAA0661
Target/Specificity RNF40 (NP_055586, 102 a.a. ~ 200 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
Dilution WB~~1:500~1000
Format Clear, colorless solution in phosphate buffered saline, pH 7.2 .
StorageStore at -20°C or lower. Aliquot to avoid repeated freezing and thawing.
PrecautionsRNF40 Antibody (monoclonal) (M09) is for research use only and not for use in diagnostic or therapeutic procedures.
Research Areas
Citations (0)

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The protein encoded by this gene contains a RING finger, a motif known to be involved in protein-protein and protein-DNA interactions. This protein was reported to interact with the tumor suppressor protein RB1. Studies of the rat counterpart suggested that this protein may function as an E3 ubiquitin-protein ligase, and facilitate the ubiquitination and degradation of syntaxin 1, which is an essential component of the neurotransmitter release machinery.


Defining the human deubiquitinating enzyme interaction landscape. Sowa ME, et al. Cell, 2009 Jul 23. PMID 19615732.RAD6-Mediated transcription-coupled H2B ubiquitylation directly stimulates H3K4 methylation in human cells. Kim J, et al. Cell, 2009 May 1. PMID 19410543.Global, in vivo, and site-specific phosphorylation dynamics in signaling networks. Olsen JV, et al. Cell, 2006 Nov 3. PMID 17081983.Monoubiquitination of human histone H2B: the factors involved and their roles in HOX gene regulation. Zhu B, et al. Mol Cell, 2005 Nov 23. PMID 16307923.Signal sequence and keyword trap in silico for selection of full-length human cDNAs encoding secretion or membrane proteins from oligo-capped cDNA libraries. Otsuki T, et al. DNA Res, 2005. PMID 16303743.

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$ 315.00
Cat# AT3676a
(40 western blots)
Availability: 7-10 days
Bulk Size
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