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RNF6 Antibody (monoclonal) (M01)

Mouse monoclonal antibody raised against a partial recombinant RNF6.

  • WB - RNF6 Antibody (monoclonal) (M01) AT3678a
    Antibody Reactive Against Recombinant Protein.Western Blot detection against Immunogen (36.74 KDa) .
  • IF - RNF6 Antibody (monoclonal) (M01) AT3678a
    Immunofluorescence of monoclonal antibody to RNF6 on HeLa cell . [antibody concentration 10 ug/ml]
  • E - RNF6 Antibody (monoclonal) (M01) AT3678a
    Detection limit for recombinant GST tagged RNF6 is approximately 0.03ng/ml as a capture antibody.
Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary Accession Q9Y252
Other Accession NM_005977
Reactivity Human
Host mouse
Clonality Monoclonal
Isotype IgG2a Kappa
Clone Names 3B1
Calculated MW 78091 Da
Additional Information
Gene ID 6049
Other Names E3 ubiquitin-protein ligase RNF6, 632-, RNF6, SPG2
Target/Specificity RNF6 (NP_005968, 1 a.a. ~ 100 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
Dilution WB~~1:500~1000
Format Clear, colorless solution in phosphate buffered saline, pH 7.2 .
StorageStore at -20°C or lower. Aliquot to avoid repeated freezing and thawing.
PrecautionsRNF6 Antibody (monoclonal) (M01) is for research use only and not for use in diagnostic or therapeutic procedures.
Research Areas
Citations (0)

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The protein encoded by this gene contains a RING-H2 finger motif. Deletions and mutations in this gene were detected in esophageal squamous cell carcinoma (ESCC), suggesting that this protein may be a potential tumor suppressor. Studies of the mouse counterpart suggested a role of this protein in the transcription regulation that controls germinal differentiation. Multiple alternatively spliced transcript variants encoding the same protein are observed.


Regulation of androgen receptor transcriptional activity and specificity by RNF6-induced ubiquitination. Xu K, et al. Cancer Cell, 2009 Apr 7. PMID 19345326.Cloning and identification of a novel RNF6 transcriptional splice variant Spg2 in human development. Zhu L, et al. Sci China C Life Sci, 2008 Apr. PMID 18368307.The ubiquitin ligase Rnf6 regulates local LIM kinase 1 levels in axonal growth cones. Tursun B, et al. Genes Dev, 2005 Oct 1. PMID 16204183.The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC). Gerhard DS, et al. Genome Res, 2004 Oct. PMID 15489334.Generation and initial analysis of more than 15,000 full-length human and mouse cDNA sequences. Strausberg RL, et al. Proc Natl Acad Sci U S A, 2002 Dec 24. PMID 12477932.

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$ 315.00
Cat# AT3678a
(40 western blots)
Availability: 7-10 days
Bulk Size
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