RPL10A Antibody (monoclonal) (M01)
Mouse monoclonal antibody raised against a partial recombinant RPL10A.
|Application ||WB, IHC, IF|
|Reactivity||Human, Mouse, Rat|
|Calculated MW||24831 Da|
|Other Names||60S ribosomal protein L10a, CSA-19, Neural precursor cell expressed developmentally down-regulated protein 6, NEDD-6, RPL10A, NEDD6|
|Target/Specificity||RPL10A (NP_009035, 108 a.a. ~ 217 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.|
|Format||Clear, colorless solution in phosphate buffered saline, pH 7.2 .|
|Storage||Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.|
|Precautions||RPL10A Antibody (monoclonal) (M01) is for research use only and not for use in diagnostic or therapeutic procedures.|
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Provided below are standard protocols that you may find useful for product applications.
Ribosomes, the organelles that catalyze protein synthesis, consist of a small 40S subunit and a large 60S subunit. Together these subunits are composed of 4 RNA species and approximately 80 structurally distinct proteins. This gene encodes a ribosomal protein that is a component of the 60S subunit. The protein belongs to the L1P family of ribosomal proteins. It is located in the cytoplasm. The expression of this gene is downregulated in the thymus by cyclosporin-A (CsA), an immunosuppressive drug. Studies in mice have shown that the expression of the ribosomal protein L10a gene is downregulated in neural precursor cells during development. This gene previously was referred to as NEDD6 (neural precursor cell expressed, developmentally downregulated 6), but it has been renamed RPL10A (ribosomal protein 10a). As is typical for genes encoding ribosomal proteins, there are multiple processed pseudogenes of this gene dispersed through the genome.
The metastasis efficiency modifier ribosomal RNA processing 1 homolog B (RRP1B) is a chromatin-associated factor. Crawford NP, et al. J Biol Chem, 2009 Oct 16. PMID 19710015. Shugoshin collaborates with protein phosphatase 2A to protect cohesin. Kitajima TS, et al. Nature, 2006 May 4. PMID 16541025. Nucleolar proteome dynamics. Andersen JS, et al. Nature, 2005 Jan 6. PMID 15635413. The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC). Gerhard DS, et al. Genome Res, 2004 Oct. PMID 15489334. Sequence comparison of human and mouse genes reveals a homologous block structure in the promoter regions. Suzuki Y, et al. Genome Res, 2004 Sep. PMID 15342556.
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