RUNX1T1 Antibody (monoclonal) (M01)
Mouse monoclonal antibody raised against a partial recombinant RUNX1T1.
|Application ||WB, IF, E|
|Calculated MW||67566 Da|
|Other Names||Protein CBFA2T1, Cyclin-D-related protein, Eight twenty one protein, Protein ETO, Protein MTG8, Zinc finger MYND domain-containing protein 2, RUNX1T1, AML1T1, CBFA2T1, CDR, ETO, MTG8, ZMYND2|
|Target/Specificity||RUNX1T1 (NP_004340, 416 a.a. ~ 525 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.|
|Format||Clear, colorless solution in phosphate buffered saline, pH 7.2 .|
|Storage||Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.|
|Precautions||RUNX1T1 Antibody (monoclonal) (M01) is for research use only and not for use in diagnostic or therapeutic procedures.|
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Provided below are standard protocols that you may find useful for product applications.
The protein encoded by this gene is a putative zinc finger transcription factor and oncoprotein. In acute myeloid leukemia, especially in the M2 subtype, the t(8;21)(q22;q22) translocation is one of the most frequent karyotypic abnormalities. The translocation produces a chimeric gene made up of the 5'-region of the RUNX1 gene fused to the 3'-region of this gene. The chimeric protein is thought to associate with the nuclear corepressor/histone deacetylase complex to block hematopoietic differentiation. Several transcript variants encoding multiple isoforms have been found for this gene.
The leukemia associated ETO nuclear repressor gene is regulated by the GATA-1 transcription factor in erythroid/megakaryocytic cells. Ajore R, et al. BMC Mol Biol, 2010 May 20. PMID 20487545.Dimer-tetramer transition controls RUNX1/ETO leukemogenic activity. Wichmann C, et al. Blood, 2010 Jul 29. PMID 20430957.NHR4 domain mutations of ETO are probably very infrequent in AML1-ETO positive myeloid leukemia cells. Hackanson B, et al. Leukemia, 2010 Apr. PMID 20090777.AML1-ETO9a is correlated with C-KIT overexpression/mutations and indicates poor disease outcome in t(8;21) acute myeloid leukemia-M2. Jiao B, et al. Leukemia, 2009 Sep. PMID 19458628.RUNX1 and its fusion oncoprotein derivative, RUNX1-ETO, induce senescence-like growth arrest independently of replicative stress. Wolyniec K, et al. Oncogene, 2009 Jul 9. PMID 19448675.
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