Register or Login
  • All
  • Uniprot Id
  • Catalog #
  • Peptide Sequence
>   home   >   Products   >   Primary Antibodies   >   Cancer   >   RUNX1T1 Antibody (monoclonal) (M01)   

RUNX1T1 Antibody (monoclonal) (M01)

Mouse monoclonal antibody raised against a partial recombinant RUNX1T1.

  • WB - RUNX1T1 Antibody (monoclonal) (M01) AT3740a
    Western Blot analysis of RUNX1T1 expression in transfected 293T cell line by RUNX1T1 monoclonal antibody (M01), clone 5A12.

    Lane 1: RUNX1T1 transfected lysate(67.566 KDa).
    Lane 2: Non-transfected lysate.
  • IF - RUNX1T1 Antibody (monoclonal) (M01) AT3740a
    Immunofluorescence of monoclonal antibody to RUNX1T1 on HeLa cell . [antibody concentration 10 ug/ml]
  • E - RUNX1T1 Antibody (monoclonal) (M01) AT3740a
    Detection limit for recombinant GST tagged RUNX1T1 is approximately 0.3ng/ml as a capture antibody.
Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary Accession Q06455
Other Accession NM_004349
Reactivity Human
Host mouse
Clonality Monoclonal
Isotype IgG2a Kappa
Clone Names 5A12
Calculated MW 67566 Da
Additional Information
Gene ID 862
Other Names Protein CBFA2T1, Cyclin-D-related protein, Eight twenty one protein, Protein ETO, Protein MTG8, Zinc finger MYND domain-containing protein 2, RUNX1T1, AML1T1, CBFA2T1, CDR, ETO, MTG8, ZMYND2
Target/Specificity RUNX1T1 (NP_004340, 416 a.a. ~ 525 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
Dilution WB~~1:500~1000
Format Clear, colorless solution in phosphate buffered saline, pH 7.2 .
StorageStore at -20°C or lower. Aliquot to avoid repeated freezing and thawing.
PrecautionsRUNX1T1 Antibody (monoclonal) (M01) is for research use only and not for use in diagnostic or therapeutic procedures.
Research Areas
Citations (0)

Thousands of laboratories across the world have published research that depended on the performance of antibodies from Abgent to advance their research. Check out links to articles that cite our products in major peer-reviewed journals, organized by research category.

Submit your citation using an Abgent antibody to, and receive a free "I Love Antibodies" mug.


The protein encoded by this gene is a putative zinc finger transcription factor and oncoprotein. In acute myeloid leukemia, especially in the M2 subtype, the t(8;21)(q22;q22) translocation is one of the most frequent karyotypic abnormalities. The translocation produces a chimeric gene made up of the 5'-region of the RUNX1 gene fused to the 3'-region of this gene. The chimeric protein is thought to associate with the nuclear corepressor/histone deacetylase complex to block hematopoietic differentiation. Several transcript variants encoding multiple isoforms have been found for this gene.


The leukemia associated ETO nuclear repressor gene is regulated by the GATA-1 transcription factor in erythroid/megakaryocytic cells. Ajore R, et al. BMC Mol Biol, 2010 May 20. PMID 20487545.Dimer-tetramer transition controls RUNX1/ETO leukemogenic activity. Wichmann C, et al. Blood, 2010 Jul 29. PMID 20430957.NHR4 domain mutations of ETO are probably very infrequent in AML1-ETO positive myeloid leukemia cells. Hackanson B, et al. Leukemia, 2010 Apr. PMID 20090777.AML1-ETO9a is correlated with C-KIT overexpression/mutations and indicates poor disease outcome in t(8;21) acute myeloid leukemia-M2. Jiao B, et al. Leukemia, 2009 Sep. PMID 19458628.RUNX1 and its fusion oncoprotein derivative, RUNX1-ETO, induce senescence-like growth arrest independently of replicative stress. Wolyniec K, et al. Oncogene, 2009 Jul 9. PMID 19448675.

Abgent welcomes feedback from its customers.

If you have used an Abgent product and would like to share how it has performed, please click on the "Submit Review" button and provide the requested information. Our staff will examine and post your review and contact you if needed.

If you have any additional inquiries please email technical services at

$ 315.00
Cat# AT3740a
(40 western blots)
Availability: 7-10 days
Bulk Size
Seasonal Special on Bulk Order
Request Quote Here

Ordering Information

United States
AlbaniaAustraliaAustriaBelgiumBosnia & HerzegovinaBrazilBulgariaCanadaChinaCroatiaCyprusCzech RepublicDenmarkEstoniaFinlandFranceGermanyGreeceHong KongHungaryIcelandIndiaIndonesiaIrelandIsraelItalyJapanKoreaLatviaLithuaniaLuxembourgMacedoniaMalaysiaMaltaNetherlandsNew ZealandNorwayPakistanPolandPortugalRomaniaSerbiaSingaporeSlovakiaSloveniaSouth AfricaSpainSwedenSwitzerlandTaiwanTurkeyUnited KingdomUnited StatesVietnamOthers
Abgent, Inc.
(888) 735-7227 / (858) 622-0099
(858) 622-0609

Shipping Information

Domestic orders (in stock items)
Shipped out the same day. Orders placed after 1 PM (PST) will ship out the next business day.
International orders
Contact your local distributors
Santa Cruz Alternative
Terms & Conditions