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>   home   >   Products   >   Primary Antibodies   >   Signal Transduction   >   RUNX2 Antibody (monoclonal) (M01)   

RUNX2 Antibody (monoclonal) (M01)

Mouse monoclonal antibody raised against a partial recombinant RUNX2.

  • WB - RUNX2 Antibody (monoclonal) (M01) AT3741a
    Antibody Reactive Against Recombinant Protein.Western Blot detection against Immunogen (36.74 KDa) .
  • WB - RUNX2 Antibody (monoclonal) (M01) AT3741a
    RUNX2 monoclonal antibody (M01), clone 1D8. Western Blot analysis of RUNX2 expression in Jurkat.
  • WB - RUNX2 Antibody (monoclonal) (M01) AT3741a
    Western Blot analysis of RUNX2 expression in transfected 293T cell line by RUNX2 monoclonal antibody (M01), clone 1D8.

    Lane 1: RUNX2 transfected lysate (Predicted MW: 60.28 KDa).
    Lane 2: Non-transfected lysate.
  • IHC - RUNX2 Antibody (monoclonal) (M01) AT3741a
    Immunoperoxidase of monoclonal antibody to RUNX2 on formalin-fixed paraffin-embedded human placenta. [antibody concentration 3 ug/ml]
  • IF - RUNX2 Antibody (monoclonal) (M01) AT3741a
    Immunofluorescence of monoclonal antibody to RUNX2 on U-2 OS cell . [antibody concentration 10 ug/ml]
  • E - RUNX2 Antibody (monoclonal) (M01) AT3741a
    Detection limit for recombinant GST tagged RUNX2 is approximately 0.03ng/ml as a capture antibody.
Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary Accession Q13950
Other Accession NM_004348
Reactivity Human
Host mouse
Clonality Monoclonal
Isotype IgG2b
Clone Names 1D8
Calculated MW 56648 Da
Additional Information
Gene ID 860
Other Names Runt-related transcription factor 2, Acute myeloid leukemia 3 protein, Core-binding factor subunit alpha-1, CBF-alpha-1, Oncogene AML-3, Osteoblast-specific transcription factor 2, OSF-2, Polyomavirus enhancer-binding protein 2 alpha A subunit, PEA2-alpha A, PEBP2-alpha A, SL3-3 enhancer factor 1 alpha A subunit, SL3/AKV core-binding factor alpha A subunit, RUNX2, AML3, CBFA1, OSF2, PEBP2A
Target/Specificity RUNX2 (NP_004339, 251 a.a. ~ 350 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
Dilution WB~~1:500~1000
Format Clear, colorless solution in phosphate buffered saline, pH 7.2 .
StorageStore at -20°C or lower. Aliquot to avoid repeated freezing and thawing.
PrecautionsRUNX2 Antibody (monoclonal) (M01) is for research use only and not for use in diagnostic or therapeutic procedures.
Research Areas
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This gene is a member of the RUNX family of transcription factors and encodes a nuclear protein with an Runt DNA-binding domain. This protein is essential for osteoblastic differentiation and skeletal morphogenesis and acts as a scaffold for nucleic acids and regulatory factors involved in skeletal gene expression. The protein can bind DNA both as a monomer or, with more affinity, as a subunit of a heterodimeric complex. Mutations in this gene have been associated with the bone development disorder cleidocranial dysplasia (CCD). Transcript variants that encode different protein isoforms result from the use of alternate promoters as well as alternate splicing.


1.Processing large-diameter poly(L-lactic acid) microfiber mesh/mesenchymal stromal cell constructs via resin embedding: an efficient histologic method.D'Alessandro D, Pertici G, Moscato S, Metelli MR, Danti S, Nesti C, Berrettini S, Petrini M, Danti S.Biomed Mater. 2014 Aug;9(4):045007. doi: 10.1088/1748-6041/9/4/045007. Epub 2014 Jul 16.2.Runt-related transcription factor 2 (RUNX2) in human colon carcinoma: A potent prognostic factor associated with estrogen receptor.Sase T, Suzuki T, Miura K, Shiiba K, Sato I, Nakamura Y, Takagi K, Onodera Y, Miki Y, Watanabe M, Ishida K, Ohnuma S, Sasaki H, Sato R, Karasawa H, Shibata C, Unno M, Sasaki I, Sasano H.Int J Cancer. 2012 Mar 7. doi: 10.1002/ijc.27525. [Epub ahead of print]

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$ 315.00
Cat# AT3741a
(40 western blots)
Availability: 7-10 days
Bulk Size
Seasonal Special on Bulk Order
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