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SEP10 Antibody (monoclonal) (M01)

Mouse monoclonal antibody raised against a full length recombinant SEPT10.

     
  • IF - SEP10 Antibody (monoclonal) (M01) AT3810a
    Immunofluorescence of monoclonal antibody to SEPT10 on HeLa cell. [antibody concentration 10 ug/ml]
    detail
  • WB - SEP10 Antibody (monoclonal) (M01) AT3810a
    Antibody Reactive Against Recombinant Protein.Western Blot detection against Immunogen (75.68 KDa) .
    detail
  • WB - SEP10 Antibody (monoclonal) (M01) AT3810a
    SEPT10 monoclonal antibody (M01), clone 2A12 Western Blot analysis of SEPT10 expression in U-2 OS ( (Cat # AT3810a )
    detail
  • WB - SEP10 Antibody (monoclonal) (M01) AT3810a
    Western Blot analysis of SEPT10 expression in transfected 293T cell line by SEPT10 monoclonal antibody (M01), clone 2A12.

    Lane 1: SEPT10 transfected lysate(52.6 KDa).
    Lane 2: Non-transfected lysate.
    detail
  • E - SEP10 Antibody (monoclonal) (M01) AT3810a
    Detection limit for recombinant GST tagged SEPT10 is approximately 1ng/ml as a capture antibody.
    detail
  • SPECIFICATION
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
WB, IF, E
Primary Accession Q9P0V9
Other Accession BC020502
Reactivity Human
Host mouse
Clonality Monoclonal
Isotype IgG1 Kappa
Clone Names 2A12
Calculated MW 52593 Da
Additional Information
Gene ID 151011
Other Names Septin-10, SEPT10
Target/Specificity SEPT10 (AAH20502, 1 a.a. ~ 454 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
Dilution WB~~1:500~1000
Format Clear, colorless solution in phosphate buffered saline, pH 7.2 .
StorageStore at -20°C or lower. Aliquot to avoid repeated freezing and thawing.
PrecautionsSEP10 Antibody (monoclonal) (M01) is for research use only and not for use in diagnostic or therapeutic procedures.
Research Areas
Citations (0)
citation

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Background

This gene encodes a member of the septin family of cytoskeletal proteins with GTPase activity. This protein localizes to the cytoplasm and nucleus and displays GTP-binding and GTPase activity. Alternate splicing results in two transcript variants encoding different isoforms.

References

1.Identification of a novel role of Septin 10 in paclitaxel-resistance in cancers through a functional genomics screen.Xu M, Takanashi M, Oikawa K, Nishi H, Isaka K, Yoshimoto T, Ohyashiki J, Kuroda M.Cancer Sci. 2012 Apr;103(4):821-7. doi: 10.1111/j.1349-7006.2012.02221.x. Epub 2012 Mar 4.

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$ 350.00
Cat# AT3810a
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