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SESN2 Antibody (monoclonal) (M03)

Mouse monoclonal antibody raised against a full length recombinant SESN2.

     
  • IF - SESN2 Antibody (monoclonal) (M03) AT3830a
    Immunofluorescence of monoclonal antibody to SES2 on HeLa cell. [antibody concentration 10 ug/ml]
    detail
  • IP - SESN2 Antibody (monoclonal) (M03) AT3830a
    Immunoprecipitation of SESN2 transfected lysate using anti-SESN2 monoclonal antibody and Protein A Magnetic Bead (U0007), and immunoblotted with SESN2 MaxPab rabbit polyclonal antibody.
    detail
  • WB - SESN2 Antibody (monoclonal) (M03) AT3830a
    Antibody Reactive Against Recombinant Protein.Western Blot detection against Immunogen (78.54 KDa) .
    detail
  • WB - SESN2 Antibody (monoclonal) (M03) AT3830a
    Western Blot analysis of SESN2 expression in transfected 293T cell line by SESN2 monoclonal antibody (M03), clone 3B8.

    Lane 1: SESN2 transfected lysate(54.5 KDa).
    Lane 2: Non-transfected lysate.
    detail
  • IHC - SESN2 Antibody (monoclonal) (M03) AT3830a
    Immunoperoxidase of monoclonal antibody to SESN2 on formalin-fixed paraffin-embedded human ovary, clear cell carcinoma. [antibody concentration 3 ug/ml]
    detail
  • E - SESN2 Antibody (monoclonal) (M03) AT3830a
    Detection limit for recombinant GST tagged SESN2 is approximately 0.3ng/ml as a capture antibody.
    detail
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
WB, IHC, IF, IP, E
Primary Accession P58004
Other Accession BC013304
Reactivity Human
Host mouse
Clonality Monoclonal
Isotype IgG2a Kappa
Clone Names 3B8
Calculated MW 54494 Da
Additional Information
Gene ID 83667
Other Names Sestrin-2, Hi95, SESN2, SEST2
Target/Specificity SESN2 (AAH13304.1, 1 a.a. ~ 480 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
Dilution WB~~1:500~1000
Format Clear, colorless solution in phosphate buffered saline, pH 7.2 .
StorageStore at -20°C or lower. Aliquot to avoid repeated freezing and thawing.
PrecautionsSESN2 Antibody (monoclonal) (M03) is for research use only and not for use in diagnostic or therapeutic procedures.
Research Areas
Citations (0)
citation

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Background

This gene encodes a member of the sestrin family of PA26-related proteins. The encoded protein may function in the regulation of cell growth and survival. This protein may be involved in cellular response to different stress conditions.

References

1.The Inhibition of Autophagy Sensitises Colon Cancer Cells with Wild-Type p53 but Not Mutant p53 to Topotecan Treatment.Li DD, Sun T, Wu XQ, Chen SP, Deng R, Jiang S, Feng GK, Pan JX, Zhang XS, Zeng YX, Zhu XF.PLoS One. 2012;7(9):e45058. Epub 2012 Sep 14.2.Association of platelet-derived growth factor receptor accumulation with increased oxidative stress and cellular injury in sestrin 2 silenced human glioblastoma cells.Liu SY, Lee YJ, Lee TC.FEBS Lett. 2011 Apr 27. [Epub ahead of print]

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$ 350.00
Cat# AT3830a
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