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>   home   >   Products   >   Primary Antibodies   >   Signal Transduction   >   SGK Antibody (monoclonal) (M01)   

SGK Antibody (monoclonal) (M01)

Mouse monoclonal antibody raised against a full length recombinant SGK.

  • WB - SGK Antibody (monoclonal) (M01) AT3854a
    Antibody Reactive Against Recombinant Protein.Western Blot detection against Immunogen (73.04 KDa) .
  • WB - SGK Antibody (monoclonal) (M01) AT3854a
    Western Blot analysis of SGK expression in transfected 293T cell line by SGK monoclonal antibody (M01), clone 4D7-G3.

    Lane 1: SGK transfected lysate(48.9 KDa).
    Lane 2: Non-transfected lysate.
  • IHC - SGK Antibody (monoclonal) (M01) AT3854a
    Immunoperoxidase of monoclonal antibody to SGK on formalin-fixed paraffin-embedded human kidney. [antibody concentration 3 ug/ml]
  • IHC - SGK Antibody (monoclonal) (M01) AT3854a
    Immunoperoxidase of monoclonal antibody to SGK on formalin-fixed paraffin-embedded human stomach tissue. [antibody concentration 5 ug/ml]
  • IF - SGK Antibody (monoclonal) (M01) AT3854a
    Immunofluorescence of monoclonal antibody to SGK on HeLa cell. [antibody concentration 10 ug/ml]
  • E - SGK Antibody (monoclonal) (M01) AT3854a
    Detection limit for recombinant GST tagged SGK is approximately 1ng/ml as a capture antibody.
  • WB - SGK Antibody (monoclonal) (M01) AT3854a
    Western blot analysis of SGK over-expressed 293 cell line, cotransfected with SGK Validated Chimera RNAi ( (Cat # AT3854a )
Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary Accession O00141
Other Accession BC001263
Reactivity Human
Host mouse
Clonality Monoclonal
Isotype IgG1 kappa
Clone Names 4D7-G3
Calculated MW 48942 Da
Additional Information
Gene ID 6446
Other Names Serine/threonine-protein kinase Sgk1, Serum/glucocorticoid-regulated kinase 1, SGK1, SGK
Target/Specificity SGK (AAH01263.1, 1 a.a. ~ 431 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
Dilution WB~~1:500~1000
Format Clear, colorless solution in phosphate buffered saline, pH 7.2 .
StorageStore at -20°C or lower. Aliquot to avoid repeated freezing and thawing.
PrecautionsSGK Antibody (monoclonal) (M01) is for research use only and not for use in diagnostic or therapeutic procedures.
Research Areas
Citations (0)

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This gene encodes a serine/threonine protein kinase that plays an important role in cellular stress response. This kinase activates certain potassium, sodium, and chloride channels, suggesting an involvement in the regulation of processes such as cell survival, neuronal excitability, and renal sodium excretion. High levels of expression of this gene may contribute to conditions such as hypertension and diabetic nephropathy. Several alternatively spliced transcript variants encoding different isoforms have been noted for this gene.


Variation at the NFATC2 Locus Increases the Risk of Thiazolinedinedione-Induced Edema in the Diabetes REduction Assessment with ramipril and rosiglitazone Medication (DREAM) Study. Bailey SD, et al. Diabetes Care, 2010 Jul 13. PMID 20628086.Serum and glucocorticoid-induced kinase (SGK) 1 and the epithelial sodium channel are regulated by multiple with no lysine (WNK) family members. Heise CJ, et al. J Biol Chem, 2010 Aug 13. PMID 20525693.Phosphopeptide screen uncovers novel phosphorylation sites of Nedd4-2 that potentiate its inhibition of the epithelial Na+ channel. Hallows KR, et al. J Biol Chem, 2010 Jul 9. PMID 20466724.Phospholipase D stabilizes HDM2 through an mTORC2/SGK1 pathway. Lyo D, et al. Biochem Biophys Res Commun, 2010 May 28. PMID 20438709.mTOR complex-2 activates ENaC by phosphorylating SGK1. Lu M, et al. J Am Soc Nephrol, 2010 May. PMID 20338997.

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$ 315.00
Cat# AT3854a
(40 western blots)
Availability: 7-10 days
Bulk Size
Seasonal Special on Bulk Order
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