SLC9A1 Antibody (monoclonal) (M01)
Mouse monoclonal antibody raised against a partial recombinant SLC9A1.
|Application ||WB, IHC, E|
|Calculated MW||90763 Da|
|Other Names||Sodium/hydrogen exchanger 1, APNH, Na(+)/H(+) antiporter, amiloride-sensitive, Na(+)/H(+) exchanger 1, NHE-1, Solute carrier family 9 member 1, SLC9A1, APNH1, NHE1|
|Target/Specificity||SLC9A1 (AAH12121, 31 a.a. ~ 130 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.|
|Format||Clear, colorless solution in phosphate buffered saline, pH 7.2 .|
|Storage||Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.|
|Precautions||SLC9A1 Antibody (monoclonal) (M01) is for research use only and not for use in diagnostic or therapeutic procedures.|
Thousands of laboratories across the world have published research that depended on the performance of antibodies from Abgent to advance their research. Check out links to articles that cite our products in major peer-reviewed journals, organized by research category.
firstname.lastname@example.org, and receive a free "I Love Antibodies" mug.
Provided below are standard protocols that you may find useful for product applications.
The Na+/H+ antiporter (SLC9A1) is a ubiquitous membrane-bound enzyme involved in pH regulation of vertebrate cells. It is specifically inhibited by the diuretic drug amiloride and activated by a variety of signals including growth factors, mitogens, neurotransmitters, tumor promoters, and others (Mattei et al., 1988 [PubMed 2846238]).
Upregulation of Na+/H+ exchanger by the AMP-activated protein kinase. Rotte A, et al. Biochem Biophys Res Commun, 2010 Aug 6. PMID 20609358.NBCn1 and NHE1 expression and activity in DeltaNErbB2 receptor-expressing MCF-7 breast cancer cells: contributions to pHi regulation and chemotherapy resistance. Lauritzen G, et al. Exp Cell Res, 2010 Sep 10. PMID 20542029.Ouabain stimulates Na-K-ATPase through a sodium/hydrogen exchanger-1 (NHE-1)-dependent mechanism in human kidney proximal tubule cells. Holthouser KA, et al. Am J Physiol Renal Physiol, 2010 Jul. PMID 20427472.Inhibition of Na(+)/H(+) exchanger 1 by 5-(N-ethyl-N-isopropyl) amiloride reduces hypoxia-induced hepatocellular carcinoma invasion and motility. Yang X, et al. Cancer Lett, 2010 Sep 28. PMID 20338684.Activation of Na+/H+ exchanger 1 by neurotensin signaling in pancreatic cancer cell lines. Olszewski U, et al. Biochem Biophys Res Commun, 2010 Mar 12. PMID 20138826.
If you have used an Abgent product and would like to share how it has performed, please click on the "Submit Review" button and provide the requested information. Our staff will examine and post your review and contact you if needed.
If you have any additional inquiries please email technical services at email@example.com.