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>   home   >   Products   >   Primary Antibodies   >   Signal Transduction   >   SMAD1 Antibody (monoclonal) (M02)   

SMAD1 Antibody (monoclonal) (M02)

Mouse monoclonal antibody raised against a partial recombinant SMAD1.

  • WB - SMAD1 Antibody (monoclonal) (M02) AT3936a
    Antibody Reactive Against Recombinant Protein.Western Blot detection against Immunogen (37.84 KDa) .
  • WB - SMAD1 Antibody (monoclonal) (M02) AT3936a
    SMAD1 monoclonal antibody (M02), clone 1D3. Western Blot analysis of SMAD1 expression in A-549 ( (Cat # AT3936a )
  • WB - SMAD1 Antibody (monoclonal) (M02) AT3936a
    SMAD1 monoclonal antibody (M02), clone 1D3 Western Blot analysis of SMAD1 expression in IMR-32 ( (Cat # AT3936a )
  • WB - SMAD1 Antibody (monoclonal) (M02) AT3936a
    SMAD1 monoclonal antibody (M02), clone 1D3. Western Blot analysis of SMAD1 expression in MES-SA/Dx5 ( (Cat # AT3936a )
  • WB - SMAD1 Antibody (monoclonal) (M02) AT3936a
    Western Blot analysis of SMAD1 expression in transfected 293T cell line by SMAD1 monoclonal antibody (M02), clone 1D3.

    Lane 1: SMAD1 transfected lysate(52.3 KDa).
    Lane 2: Non-transfected lysate.
  • IHC - SMAD1 Antibody (monoclonal) (M02) AT3936a
    Immunoperoxidase of monoclonal antibody to SMAD1 on formalin-fixed paraffin-embedded human salivary gland tissue. [antibody concentration 3 ug/ml]
  • IF - SMAD1 Antibody (monoclonal) (M02) AT3936a
    Immunofluorescence of monoclonal antibody to SMAD1 on HeLa cell. [antibody concentration 10 ug/ml]
  • E - SMAD1 Antibody (monoclonal) (M02) AT3936a
    Detection limit for recombinant GST tagged SMAD1 is approximately 3ng/ml as a capture antibody.
Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary Accession Q15797
Other Accession NM_005900
Reactivity Human
Host mouse
Clonality Monoclonal
Isotype IgG1 kappa
Clone Names 1D3
Calculated MW 52260 Da
Additional Information
Gene ID 4086
Other Names Mothers against decapentaplegic homolog 1, MAD homolog 1, Mothers against DPP homolog 1, JV4-1, Mad-related protein 1, SMAD family member 1, SMAD 1, Smad1, hSMAD1, Transforming growth factor-beta-signaling protein 1, BSP-1, SMAD1, BSP1, MADH1, MADR1
Target/Specificity SMAD1 (NP_005891, 1 a.a. ~ 110 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
Dilution WB~~1:500~1000
Format Clear, colorless solution in phosphate buffered saline, pH 7.2 .
StorageStore at -20°C or lower. Aliquot to avoid repeated freezing and thawing.
PrecautionsSMAD1 Antibody (monoclonal) (M02) is for research use only and not for use in diagnostic or therapeutic procedures.
Research Areas
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The protein encoded by this gene belongs to the SMAD, a family of proteins similar to the gene products of the Drosophila gene 'mothers against decapentaplegic' (Mad) and the C. elegans gene Sma. SMAD proteins are signal transducers and transcriptional modulators that mediate multiple signaling pathways. This protein mediates the signals of the bone morphogenetic proteins (BMPs), which are involved in a range of biological activities including cell growth, apoptosis, morphogenesis, development and immune responses. In response to BMP ligands, this protein can be phosphorylated and activated by the BMP receptor kinase. The phosphorylated form of this protein forms a complex with SMAD4, which is important for its function in the transcription regulation. This protein is a target for SMAD-specific E3 ubiquitin ligases, such as SMURF1 and SMURF2, and undergoes ubiquitination and proteasome-mediated degradation. Alternatively spliced transcript variants encoding the same protein have been observed.


Maternal genes and facial clefts in offspring: a comprehensive search for genetic associations in two population-based cleft studies from Scandinavia. Jugessur A, et al. PLoS One, 2010 Jul 9. PMID 20634891.Smad-dependent and smad-independent induction of id1 by prostacyclin analogues inhibits proliferation of pulmonary artery smooth muscle cells in vitro and in vivo. Yang J, et al. Circ Res, 2010 Jul 23. PMID 20522807.Induced expression of bone morphogenetic protein-6 and Smads signaling in human monocytes derived dendritic cells during sickle-cell pathology with orthopedic complications. Abhishek K, et al. Biochem Biophys Res Commun, 2010 Jun 11. PMID 20460105.Inflammation anergy in human intestinal macrophages is due to Smad-induced IkappaBalpha expression and NF-kappaB inactivation. Smythies LE, et al. J Biol Chem, 2010 Jun 18. PMID 20388715.Autocrine bone morphogenetic protein-9 signals through activin receptor-like kinase-2/Smad1/Smad4 to promote ovarian cancer cell proliferation. Herrera B, et al. Cancer Res, 2009 Dec 15. PMID 19996292.

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$ 315.00
Cat# AT3936a
(40 western blots)
Availability: 7-10 days
Bulk Size
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