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>   home   >   Products   >   Primary Antibodies   >   Signal Transduction   >   SMAD1 Antibody (monoclonal) (M03)   

SMAD1 Antibody (monoclonal) (M03)

Mouse monoclonal antibody raised against a full length recombinant SMAD1.

  • WB - SMAD1 Antibody (monoclonal) (M03) AT3937a
    Antibody Reactive Against Recombinant Protein.Western Blot detection against Immunogen (76.89 KDa) .
  • WB - SMAD1 Antibody (monoclonal) (M03) AT3937a
    SMAD1 monoclonal antibody (M03), clone 2E9 Western Blot analysis of SMAD1 expression in HeLa ( (Cat # AT3937a )
  • WB - SMAD1 Antibody (monoclonal) (M03) AT3937a
    SMAD1 monoclonal antibody (M03), clone 2E9. Western Blot analysis of SMAD1 expression in PC-12 ( (Cat # AT3937a )
  • WB - SMAD1 Antibody (monoclonal) (M03) AT3937a
    SMAD1 monoclonal antibody (M03), clone 2E9. Western Blot analysis of SMAD1 expression in IMR-32 ( (Cat # AT3937a )
  • WB - SMAD1 Antibody (monoclonal) (M03) AT3937a
    SMAD1 monoclonal antibody (M03), clone 2E9. Western Blot analysis of SMAD1 expression in Raw 264.7 ( (Cat # AT3937a )
  • WB - SMAD1 Antibody (monoclonal) (M03) AT3937a
    SMAD1 monoclonal antibody (M03), clone 2E9. Western Blot analysis of SMAD1 expression in NIH/3T3 ( (Cat # AT3937a )
  • IHC - SMAD1 Antibody (monoclonal) (M03) AT3937a
    Immunoperoxidase of monoclonal antibody to SMAD1 on formalin-fixed paraffin-embedded human colon. [antibody concentration 3 ug/ml]
  • IF - SMAD1 Antibody (monoclonal) (M03) AT3937a
    Immunofluorescence of monoclonal antibody to SMAD1 on HeLa cell. [antibody concentration 10 ug/ml]
  • E - SMAD1 Antibody (monoclonal) (M03) AT3937a
    Detection limit for recombinant GST tagged SMAD1 is approximately 1ng/ml as a capture antibody.
Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary Accession Q15797
Other Accession BC001878
Reactivity Human, Mouse, Rat
Host mouse
Clonality Monoclonal
Isotype IgG1 Kappa
Clone Names 2E10
Calculated MW 52260 Da
Additional Information
Gene ID 4086
Other Names Mothers against decapentaplegic homolog 1, MAD homolog 1, Mothers against DPP homolog 1, JV4-1, Mad-related protein 1, SMAD family member 1, SMAD 1, Smad1, hSMAD1, Transforming growth factor-beta-signaling protein 1, BSP-1, SMAD1, BSP1, MADH1, MADR1
Target/Specificity SMAD1 (AAH01878.1, 1 a.a. ~ 465 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
Dilution WB~~1:500~1000
Format Clear, colorless solution in phosphate buffered saline, pH 7.2 .
StorageStore at -20°C or lower. Aliquot to avoid repeated freezing and thawing.
PrecautionsSMAD1 Antibody (monoclonal) (M03) is for research use only and not for use in diagnostic or therapeutic procedures.
Research Areas
Citations (0)

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The protein encoded by this gene belongs to the SMAD, a family of proteins similar to the gene products of the Drosophila gene 'mothers against decapentaplegic' (Mad) and the C. elegans gene Sma. SMAD proteins are signal transducers and transcriptional modulators that mediate multiple signaling pathways. This protein mediates the signals of the bone morphogenetic proteins (BMPs), which are involved in a range of biological activities including cell growth, apoptosis, morphogenesis, development and immune responses. In response to BMP ligands, this protein can be phosphorylated and activated by the BMP receptor kinase. The phosphorylated form of this protein forms a complex with SMAD4, which is important for its function in the transcription regulation. This protein is a target for SMAD-specific E3 ubiquitin ligases, such as SMURF1 and SMURF2, and undergoes ubiquitination and proteasome-mediated degradation. Alternatively spliced transcript variants encoding the same protein have been observed.


1.Temporal and regional patterns of Smad activation in the rat hippocampus following global ischemia.Nakajima T, Yanagihara M, Nishii HJ Neurol Sci. 2013 Nov 19. pii: S0022-510X(13)03041-4. doi: 10.1016/j.jns.2013.11.012.

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$ 315.00
Cat# AT3937a
(40 western blots)
Availability: 7-10 days
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