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>   home   >   Products   >   Primary Antibodies   >   Signal Transduction   >   SMAD5 Antibody (monoclonal) (M03)   

SMAD5 Antibody (monoclonal) (M03)

Mouse monoclonal antibody raised against a partial recombinant SMAD5.

  • WB - SMAD5 Antibody (monoclonal) (M03) AT3945a
    Antibody Reactive Against Recombinant Protein.Western Blot detection against Immunogen (34.21 KDa) .
  • WB - SMAD5 Antibody (monoclonal) (M03) AT3945a
    SMAD5 monoclonal antibody (M03), clone 3A10 Western Blot analysis of SMAD5 expression in HeLa ( (Cat # AT3945a )
  • IF - SMAD5 Antibody (monoclonal) (M03) AT3945a
    Immunofluorescence of monoclonal antibody to SMAD5 on HeLa cell. [antibody concentration 10 ug/ml]
  • E - SMAD5 Antibody (monoclonal) (M03) AT3945a
    Detection limit for recombinant GST tagged SMAD5 is approximately 0.3ng/ml as a capture antibody.
Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary Accession Q99717
Other Accession BC009682
Reactivity Human
Host mouse
Clonality Monoclonal
Isotype IgG2a Kappa
Clone Names 3A10
Calculated MW 52258 Da
Additional Information
Gene ID 4090
Other Names Mothers against decapentaplegic homolog 5, MAD homolog 5, Mothers against DPP homolog 5, JV5-1, SMAD family member 5, SMAD 5, Smad5, hSmad5, SMAD5, MADH5
Target/Specificity SMAD5 (AAH09682, 105 a.a. ~ 182 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
Dilution WB~~1:500~1000
Format Clear, colorless solution in phosphate buffered saline, pH 7.2 .
StorageStore at -20°C or lower. Aliquot to avoid repeated freezing and thawing.
PrecautionsSMAD5 Antibody (monoclonal) (M03) is for research use only and not for use in diagnostic or therapeutic procedures.
Research Areas
Citations (0)

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Smad-dependent and smad-independent induction of id1 by prostacyclin analogues inhibits proliferation of pulmonary artery smooth muscle cells in vitro and in vivo. Yang J, et al. Circ Res, 2010 Jul 23. PMID 20522807.Alternative splicing of SMADs in differentiation and tissue homeostasis. Tao S, et al. Dev Growth Differ, 2010 May. PMID 20148926.Smad1-Smad5 ovarian conditional knockout mice develop a disease profile similar to the juvenile form of human granulosa cell tumors. Middlebrook BS, et al. Endocrinology, 2009 Dec. PMID 19819941.BMP4-Smad signaling pathway mediates adriamycin-induced premature senescence in lung cancer cells. Su D, et al. J Biol Chem, 2009 May 1. PMID 19269967.Investigation of DNA polymorphisms in SMAD genes for genetic predisposition to diabetic nephropathy in patients with type 1 diabetes mellitus. McKnight AJ, et al. Diabetologia, 2009 May. PMID 19247629.

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$ 315.00
Cat# AT3945a
(40 western blots)
Availability: 7-10 days
Bulk Size
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