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SPARC Antibody (monoclonal) (M02)

Mouse monoclonal antibody raised against a full length recombinant SPARC.

  • WB - SPARC Antibody (monoclonal) (M02) AT4014a
    Antibody Reactive Against Recombinant Protein.Western Blot detection against Immunogen (59.07 KDa) .
  • WB - SPARC Antibody (monoclonal) (M02) AT4014a
    SPARC monoclonal antibody (M02), clone 1B2. Western Blot analysis of SPARC expression in human kidney.
  • WB - SPARC Antibody (monoclonal) (M02) AT4014a
    SPARC monoclonal antibody (M02), clone 1B2 Western Blot analysis of SPARC expression in A-431 ( (Cat # AT4014a )
  • WB - SPARC Antibody (monoclonal) (M02) AT4014a
    Western Blot analysis of SPARC expression in transfected 293T cell line by SPARC monoclonal antibody (M02), clone 1B2.

    Lane 1: SPARC transfected lysate(34.6 KDa).
    Lane 2: Non-transfected lysate.
  • E - SPARC Antibody (monoclonal) (M02) AT4014a
    Detection limit for recombinant GST tagged SPARC is approximately 3ng/ml as a capture antibody.
Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary Accession P09486
Other Accession BC004974
Reactivity Human
Host mouse
Clonality Monoclonal
Isotype IgG1 Kappa
Clone Names 1B2
Calculated MW 34632 Da
Additional Information
Gene ID 6678
Other Names SPARC, Basement-membrane protein 40, BM-40, Osteonectin, ON, Secreted protein acidic and rich in cysteine, SPARC, ON
Target/Specificity SPARC (AAH04974.1, 1 a.a. ~ 303 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
Dilution WB~~1:500~1000
Format Clear, colorless solution in phosphate buffered saline, pH 7.2 .
StorageStore at -20°C or lower. Aliquot to avoid repeated freezing and thawing.
PrecautionsSPARC Antibody (monoclonal) (M02) is for research use only and not for use in diagnostic or therapeutic procedures.
Research Areas
Citations (0)

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Secreted protein acidic and rich in cysteine/osteonectin/BM40, or SPARC, is a matrix-associated protein that elicits changes in cell shape, inhibits cell-cycle progression, and influences the synthesis of extracellular matrix (ECM) (Bradshaw et al., 2003 [PubMed 12721366]).


1.The matricellular protein SPARC supports follicular dendritic cell networking toward Th17 responses.Piconese S, Costanza M, Tripodo C, Sangaletti S, Musio S, Pittoni P, Poliani PL, Burocchi A, Passafaro AL, Gorzanelli A, Vitali C, Chiodoni C, Barnaba V, Pedotti R, Colombo MP.J Autoimmun. 2011 Sep 28.

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$ 315.00
Cat# AT4014a
(40 western blots)
Availability: 7-10 days
Bulk Size
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