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SPN Antibody (monoclonal) (M01)

Mouse monoclonal antibody raised against a full length recombinant SPN.

     
  • WB - SPN Antibody (monoclonal) (M01) AT4026a
    Antibody Reactive Against Recombinant Protein.Western Blot detection against Immunogen (69.74 KDa) .
    detail
  • WB - SPN Antibody (monoclonal) (M01) AT4026a
    Western Blot analysis of SPN expression in transfected 293T cell line by SPN monoclonal antibody (M01), clone 3G8.

    Lane 1: SPN transfected lysate(59.327 KDa).
    Lane 2: Non-transfected lysate.
    detail
  • E - SPN Antibody (monoclonal) (M01) AT4026a
    Detection limit for recombinant GST tagged SPN is 0.03 ng/ml as a capture antibody.
    detail
  • SPECIFICATION
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
WB, E
Primary Accession P16150
Other Accession BC012350
Reactivity Human
Host mouse
Clonality Monoclonal
Isotype IgG2a Kappa
Clone Names 3G8
Calculated MW 40322 Da
Additional Information
Gene ID 6693
Other Names Leukosialin, Galactoglycoprotein, GALGP, Leukocyte sialoglycoprotein, Sialophorin, CD43, SPN, CD43
Target/Specificity SPN (AAH12350, 1 a.a. ~ 400 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
Dilution WB~~1:500~1000
Format Clear, colorless solution in phosphate buffered saline, pH 7.2 .
StorageStore at -20°C or lower. Aliquot to avoid repeated freezing and thawing.
PrecautionsSPN Antibody (monoclonal) (M01) is for research use only and not for use in diagnostic or therapeutic procedures.
Research Areas
Citations (0)
citation

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Background

Sialophorin (leukosialin) is a major sialoglycoprotein on the surface of human T lymphocytes, monocytes, granulocytes, and some B lymphocytes, which appears to be important for immune function and may be part of a physiologic ligand-receptor complex involved in T-cell activation.

References

Binding of the Streptococcus gordonii DL1 surface protein Hsa to the host cell membrane glycoproteins CD11b, CD43, and CD50. Urano-Tashiro Y, et al. Infect Immun, 2008 Oct. PMID 18678668.The cleavage of neutrophil leukosialin (CD43) by cathepsin G releases its extracellular domain and triggers its intramembrane proteolysis by presenilin/gamma-secretase. Mambole A, et al. J Biol Chem, 2008 Aug 29. PMID 18586676.The selective expression of CD43 in adenoid cystic carcinoma. Seethala RR, et al. Appl Immunohistochem Mol Morphol, 2008 Mar. PMID 18227725.LFA-1-mediated leukocyte adhesion regulated by interaction of CD43 with LFA-1 and CD147. Khunkaewla P, et al. Mol Immunol, 2008 Mar. PMID 17996943.Site-specific morphologic differences in extranodal marginal zone B-cell lymphomas. Rawal A, et al. Arch Pathol Lab Med, 2007 Nov. PMID 17979485.

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$ 350.00
Cat# AT4026a
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