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>   home   >   Products   >   Primary Antibodies   >   Signal Transduction   >   SQSTM1 Antibody (monoclonal) (M01)   

SQSTM1 Antibody (monoclonal) (M01)

Mouse monoclonal antibody raised against a full length recombinant SQSTM1.

  • WB - SQSTM1 Antibody (monoclonal) (M01) AT4033a
    Antibody Reactive Against Recombinant Protein.Western Blot detection against Immunogen (74.14 KDa) .
  • IF - SQSTM1 Antibody (monoclonal) (M01) AT4033a
    Immunofluorescence of monoclonal antibody to SQSTM1 on HeLa cell. [antibody concentration 10 ug/ml]
  • E - SQSTM1 Antibody (monoclonal) (M01) AT4033a
    Detection limit for recombinant GST tagged SQSTM1 is approximately 0.03ng/ml as a capture antibody.
Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary Accession Q13501
Other Accession BC003139
Reactivity Human, Mouse
Host Mouse
Clonality Monoclonal
Isotype IgG2a kappa
Clone Names 2C11
Calculated MW 47687 Da
Additional Information
Gene ID 8878
Other Names Sequestosome-1, EBI3-associated protein of 60 kDa, EBIAP, p60, Phosphotyrosine-independent ligand for the Lck SH2 domain of 62 kDa, Ubiquitin-binding protein p62, SQSTM1, ORCA, OSIL
Target/Specificity SQSTM1 (AAH03139.1, 1 a.a. ~ 440 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
Dilution WB~~1:500~1000
Format Clear, colorless solution in phosphate buffered saline, pH 7.2 .
StorageStore at -20°C or lower. Aliquot to avoid repeated freezing and thawing.
PrecautionsSQSTM1 Antibody (monoclonal) (M01) is for research use only and not for use in diagnostic or therapeutic procedures.
Research Areas
Citations ( 0 )


This gene encodes a multifunctional protein that binds ubiquitin and regulates activation of the nuclear factor kappa-B (NF-kB) signaling pathway. The protein functions as a scaffolding/adaptor protein in concert with TNF receptor-associated factor 6 to mediate activation of NF-kB in response to upstream signals. Alternatively spliced transcript variants encoding either the same or different isoforms have been identified for this gene. Mutations in this gene result in sporadic and familial Paget disease of bone.


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Epub 2014 Jan 10.6.MIR376A Is a Regulator of Starvation-Induced Autophagy.Korkmaz G, Tekirdag KA, Ozturk DG, Kosar A, Sezerman OU, Gozuacik DPLoS One. 2013 Dec 16;8(12):e82556. doi: 10.1371/journal.pone.0082556. eCollection 2013.7.Autophagy variation within a cell population determines cell fate through selective degradation of Fap-1.Gump JM, Staskiewicz L, Morgan MJ, Bamberg A, Riches DW, Thorburn ANat Cell Biol. 2014 Jan;16(1):47-54. doi: 10.1038/ncb2886. Epub 2013 Dec 8.8.Autosis is a Na+,K+-ATPase-regulated form of cell death triggered by autophagy-inducing peptides, starvation, and hypoxia-ischemia.Liu Y, Shoji-Kawata S, Sumpter RM Jr, Wei Y, Ginet V, Zhang L, Posner B, Tran KA, Green DR, Xavier RJ, Shaw SY, Clarke PG, Puyal J, Levine BProc Natl Acad Sci U S A. 2013 Dec 17;110(51):20364-71. doi: 10.1073/pnas.1319661110. Epub 2013 Nov 25.9.New Autophagy Reporter Mice Reveal Dynamics of Proximal Tubular Autophagy.Li L, Wang ZV, Hill JA, Lin FJ Am Soc Nephrol. 2013 Oct 31.10.C9orf72 frontotemporal lobar degeneration is characterised by frequent neuronal sense and antisense RNA foci.Mizielinska S, Lashley T, Norona FE, Clayton EL, Ridler CE, Fratta P, Isaacs AMActa Neuropathol. 2013 Oct 30.11.Melatonin administration decreases adipogenesis in the liver of ob/ob mice through autophagy Luxan-Delgado B, Caballero B, Potes Y, Rubio-Gonzalez A, Rodriguez I, Gutierrez-Rodriguez J, Solano JJ, Coto-Montes AJ Pineal Res. 2013 Oct 18. doi: 10.1111/jpi.12104.12.Excitotoxic glutamate insults block autophagic flux in hippocampal neurons.Kulbe JR, Mulcahy Levy JM, Coultrap SJ, Thorburn A, Ulrich Bayer K.Brain Research Volume 1542, 13 January 2014, Pages 12-1913.Autophagic-lysosomal pathway is the main proteolytic system modified in the skeletal muscle of esophageal cancer patients.Tardif N, Klaude M, Lundell L, Thorell A, Rooyackers OAm J Clin Nutr. 2013 Dec;98(6):1485-92. doi: 10.3945/ajcn.113.063859. 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Epub 2013 Feb 20.26.Site-specific Mtm1 mutagenesis by an AAV-Cre vector reveals that myotubularin is essential in adult muscle.Joubert R, Vignaud A, Le M, Moal C, Messaddeq N, Buj-Bello AHum Mol Genet. 2013 Feb 10.27.Dysregulation of autophagy in chronic lymphocytic leukemia with the small-molecule Sirtuin inhibitor Tenovin-6.Maccallum SF, Groves MJ, James J, Murray K, Appleyard V, Prescott AR, Drbal AA, Nicolaou A, Cunningham J, Haydock S, Ganley IG, Westwood NJ, Coates PJ, Lain S, Tauro SSci Rep. 2013 Feb 14;3:1275. doi: 10.1038/srep01275.28.Dopamine and its metabolites in cathepsin D heterozygous mice before and after MPTP administration.Crabtree D, Boyer-Guittaut M, Ouyang X, Fineberg N, Zhang JNeurosci Lett. 2013 Feb 4. pii: S0304-3940(13)00077-3. doi: 10.1016/j.neulet.2013.01.035.29.Host Cell Autophagy Modulates Early Stages of Adenovirus Infections in Airway Epithelial Cells.Zeng X, Carlin CR.J Virol. 2012 Dec 12.30.Astrocyte-Specific Overexpression of Nrf2 Delays Motor Pathology and Synuclein Aggregation throughout the CNS in the Alpha-Synuclein Mutant (A53T) Mouse Model.Gan L, Vargas MR, Johnson DA, Johnson JA.J Neurosci. 2012 Dec 5;32(49):17775-87. doi: 10.1523/JNEUROSCI.3049-12.2012.31.Hyperactivation of the mammalian degenerin MDEG promotes caspase-8 activation and apoptosis.Pan JA, Fan Y, Gandhirajan RK, Madesh M, Zong WX.J Biol Chem. 2012 Dec 13.32.Suppression of Autophagic Flux by Bile Acids in Hepatocytes.Manley S, Ni HM, Kong B, Apte U, Guo G, Ding WXToxicol Sci. 2013 Nov 4.33.Defective autophagy and mTORC1 signaling in myotubularin null mice.Fetalvero KM, Yu Y, Goetschkes M, Liang G, Valdez RA, Gould T, Triantafellow E, Bergling S, Loureiro J, Eash J, Lin V, Porter JA, Finan PM, Walsh K, Yang Y, Mao X, Murphy LO.Mol Cell Biol. 2012 Oct 29. 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DOI: 10.1021/cn300145z38.Tissue Specific Induction of p62/Sqstm1 by Farnesoid X Receptor.Williams JA, Thomas AM, Li G, Kong B, Zhan L, Inaba Y, Xie W, Ding WX, Guo GL.PLoS One. 2012;7(8):e43961. Epub 2012 Aug 27.39.Sorafenib has potent anti-tumor activity in multiple myeloma in vitro, ex vivo and in vivo, in the 5T33MM mouse model.Kharaziha P, De Raeve H, Fristedt C, Li Q, Gruber A, Johnsson P, Kokaraki G, Panzar M, Laane E, Osterborg A, Zhivotovsky B, Jernberg-Wiklund H, Grander D, Celsing F, Bjorkholm M, Vanderkerken K, Panaretakis T.Cancer Res. 2012 Sep 4.40.Genetic Ablation of Nrf2/Antioxidant Response Pathway in Alexander Disease Mice Reduces Hippocampal Gliosis but Does Not Impact Survival.Hagemann TL, Jobe EM, Messing A.PLoS One. 2012;7(5):e37304. Epub 2012 May 31.41.MAP1B Interaction with the FW Domain of the Autophagic Receptor Nbr1 Facilitates Its Association to the Microtubule Network.Marchbank K, Waters S, Roberts RG, Solomon E, Whitehouse CA.Int J Cell Biol. 2012;2012:208014. Epub 2012 May 10.42.Liver-specific loss of atg5 causes persistent activation of nrf2 and protects against acetaminophen-induced liver injury.Ni HM, Boggess N, McGill MR, Lebofsky M, Borude P, Apte U, Jaeschke H, Ding WX.Toxicol Sci. 2012 Jun;127(2):438-50. Epub 2012 Apr 5.43.Chronic Activation of mTOR Complex 1 Is Sufficient to Cause Hepatocellular Carcinoma in Mice.Menon S, Yecies JL, Zhang HH, Howell JJ, Nicholatos J, Harputlugil E, Bronson RT, Kwiatkowski DJ, Manning BD.Sci Signal. 2012 Mar 27;5(217):ra24.44.Progressive neuronal inclusion formation and axonal degeneration in CHMP2B mutant transgenic mice.Ghazi-Noori S, Froud KE, Mizielinska S, Powell C, Smidak M, Fernandez de Marco M, O'Malley C, Farmer M, Parkinson N, Fisher EM, Asante EA, Brandner S, Collinge J, Isaacs AM.Brain. 2012 Mar;135(Pt 3):819-32.45.A centronuclear myopathy-dynamin 2 mutation impairs autophagy in mice.Durieux AC, Vassilopoulos S, Laine J, Fraysse B, Brinas L, Prudhon B, Castells J, Freyssenet D, Bonne G, Guicheney P, Bitoun M.Traffic. 2012 Feb 28. doi: 10.1111/j.1600-0854.2012.01348.x. [Epub ahead of print]46.Targeting of distinct signaling cascades and cancer-associated fibroblasts define the efficacy of Sorafenib against prostate cancer cells.Kharaziha P, Rodriguez P, Li Q, Rundqvist H, Bjorklund AC, Augsten M, Ullen A, Egevad L, Wiklund P, Nilsson S, Kroemer G, Grander D, Panaretakis T.Cell Death Dis. 2012 Jan 26;3:e262. doi: 10.1038/cddis.2012.1.47.Class III PI3K Vps34 plays an essential role in autophagy and in heart and liver function.Jaber N, Dou Z, Chen JS, Catanzaro J, Jiang YP, Ballou LM, Selinger E, Ouyang X, Lin RZ, Zhang J, Zong WX.Proc Natl Acad Sci U S A. 2012 Feb 7;109(6):2003-8. Epub 2012 Jan 23.48.Cathepsin Cleavage of Sirtuin-1 in Endothelial Progenitor Cells Mediates Stress-Induced Premature Senescence.Chen J, Xavier S, Moskowitz-Kassai E, Chen R, Lu CY, Sanduski K, Spes A, Turk B, Goligorsky MS.Am J Pathol. 2012 Jan 9. [Epub ahead of print]49.Autophagic degradation of tau in primary neurons and its enhancement by trehalose.Kruger U, Wang Y, Kumar S, Mandelkow EM.Neurobiol Aging. 2011 Dec 12.50.Mutations in UBQLN2 cause dominant X-linked juvenile and adult-onset ALS and ALS/dementia.Deng HX, Chen W, Hong ST, Boycott KM, Gorrie GH, Siddique N, Yang Y, Fecto F, Shi Y, Zhai H, Jiang H, Hirano M, Rampersaud E, Jansen GH, Donkervoort S, Bigio EH, Brooks BR, Ajroud K, Sufit RL, Haines JL, Mugnaini E, Pericak-Vance MA, Siddique T.Nature. 2011 Aug 21;477(7363):211-5. doi: 10.1038/nature10353.51.Modulation of pediatric brain tumor autophagy and chemosensitivity.Levy JM, Thorburn A.J Neurooncol. 2011 Aug 13. [Epub ahead of print]52.Autophagy promotes T-cell survival through degradation of proteins of the cell death machinery.Kovacs JR, Li C, Yang Q, Li G, Garcia IG, Ju S, Roodman DG, Windle JJ, Zhang X, Lu B.Cell Death Differ. 2011 Jun 10. doi: 10.1038/cdd.2011.78. [Epub ahead of print]53.Pancreatic cancers require autophagy for tumor growth.Yang S, Wang X, Contino G, Liesa M, Sahin E, Ying H, Bause A, Li Y, Stommel JM, Dell'antonio G, Mautner J, Tonon G, Haigis M, Shirihai OS, Doglioni C, Bardeesy N, Kimmelman AC.Genes Dev. 2011 Apr 1;25(7):717-29. Epub 2011 Mar 15.54.Neighbor of Brca1 gene (Nbr1) functions as a negative regulator of postnatal osteoblastic bone formation and p38 MAPK activity.Whitehouse CA, Waters S, Marchbank K, Horner A, McGowan NW, V Jovanovic J, Xavier GM, Kashima TG, Cobourne MT, Richards GO, Sharpe PT, Skerry TM, Grigoriadis AE, Solomon E.Proc Natl Acad Sci U S A. 2010 Jun 29. 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$ 315.00
Cat# AT4033a
(40 western blots)
Availability: 7-10 days
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