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>   home   >   Products   >   Primary Antibodies   >   Signal Transduction   >   SSH3 Antibody (monoclonal) (M01)   

SSH3 Antibody (monoclonal) (M01)

Mouse monoclonal antibody raised against a partial recombinant SSH3.

  • WB - SSH3 Antibody (monoclonal) (M01) AT4046a
    Antibody Reactive Against Recombinant Protein.Western Blot detection against Immunogen (36.63 KDa) .
  • WB - SSH3 Antibody (monoclonal) (M01) AT4046a
    SSH3 monoclonal antibody (M01), clone 6F9 Western Blot analysis of SSH3 expression in A-431 ( (Cat # AT4046a )
  • WB - SSH3 Antibody (monoclonal) (M01) AT4046a
    Western Blot analysis of SSH3 expression in transfected 293T cell line by SSH3 monoclonal antibody (M01), clone 6F9.

    Lane 1: SSH3 transfected lysate(73 KDa).
    Lane 2: Non-transfected lysate.
  • IHC - SSH3 Antibody (monoclonal) (M01) AT4046a
    Immunoperoxidase of monoclonal antibody to SSH3 on formalin-fixed paraffin-embedded human salivary gland. [antibody concentration 1 ~ 10 ug/ml]
  • IF - SSH3 Antibody (monoclonal) (M01) AT4046a
    Immunofluorescence of monoclonal antibody to SSH3 on A-431 cell. [antibody concentration 10 ug/ml]
  • IP - SSH3 Antibody (monoclonal) (M01) AT4046a
    Immunoprecipitation of SSH3 transfected lysate using anti-SSH3 monoclonal antibody and Protein A Magnetic Bead (U0007), and immunoblotted with SSH3 monoclonal antibody.
  • E - SSH3 Antibody (monoclonal) (M01) AT4046a
    Detection limit for recombinant GST tagged SSH3 is approximately 0.3ng/ml as a capture antibody.
Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary Accession Q8TE77
Other Accession NM_017857
Reactivity Human
Host mouse
Clonality Monoclonal
Isotype IgG2a Kappa
Clone Names 6F9
Calculated MW 72996 Da
Additional Information
Gene ID 54961
Other Names Protein phosphatase Slingshot homolog 3, SSH-like protein 3, SSH-3L, hSSH-3L, SSH3, SSH3L
Target/Specificity SSH3 (NP_060327, 293 a.a. ~ 391 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
Dilution WB~~1:500~1000
Format Clear, colorless solution in phosphate buffered saline, pH 7.2 .
StorageStore at -20°C or lower. Aliquot to avoid repeated freezing and thawing.
PrecautionsSSH3 Antibody (monoclonal) (M01) is for research use only and not for use in diagnostic or therapeutic procedures.
Research Areas
Citations (0)

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The ADF (actin-depolymerizing factor)/cofilin family (see MIM 601442) is composed of stimulus-responsive mediators of actin dynamics. ADF/cofilin proteins are inactivated by kinases such as LIM domain kinase-1 (LIMK1; MIM 601329). The SSH family appears to play a role in actin dynamics by reactivating ADF/cofilin proteins in vivo (Niwa et al., 2002 [PubMed 11832213]).


Slingshot-3 dephosphorylates ADF/cofilin but is dispensable for mouse development. Kousaka K, et al. Genesis, 2008 May. PMID 18442045.The slingshot family of phosphatases mediates Rac1 regulation of cofilin phosphorylation, laminin-332 organization, and motility behavior of keratinocytes. Kligys K, et al. J Biol Chem, 2007 Nov 2. PMID 17848544.A probability-based approach for high-throughput protein phosphorylation analysis and site localization. Beausoleil SA, et al. Nat Biotechnol, 2006 Oct. PMID 16964243.Diversification of transcriptional modulation: large-scale identification and characterization of putative alternative promoters of human genes. Kimura K, et al. Genome Res, 2006 Jan. PMID 16344560.Interplay between components of a novel LIM kinase-slingshot phosphatase complex regulates cofilin. Soosairajah J, et al. EMBO J, 2005 Feb 9. PMID 15660133.

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$ 315.00
Cat# AT4046a
(40 western blots)
Availability: 7-10 days
Bulk Size
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